Cloning and characterization of the promoter region of human LPTS/PinX1 gene
文献类型：期刊论文
| 作者 | Wang, SQ; Liao, C; Li, TP; Zhao, MJ |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
 |
| 出版日期 | 2004 |
| 卷号 | 1676期号:3页码:261-265 |
| 关键词 | LPTS/PinX1 transcription promoter luciferase assay DNase I hypersensitive site |
| 通讯作者 | Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,mjzhao@sibs.ac.cn |
| 英文摘要 | The gene for LPTS/PinX1 encodes a potent telomerase inhibitor and suppresses tumor cell growth. In order to investigate the transcriptional regulation of this gene, we isolated its 5'-flanking region from the human genomic BAC clone and identified a major transcriptional initiation site. The sequence of the 5'-flanking region is GC-rich, lacks canonical TATA box, but contains potential binding sites for a variety of transcription factors. The deletion analysis indicated that the proximal 100 bp (from nt - 66 to + 34) is essential for minimal promoter activity and the regions of promoter from nt - 1272 to - 573 and in - 330 to - 66 are required for maximal expression of the LPTS/PinX1 gene. Four DNase I hypersensitive sites (DHS1-4) mapping to the regions of transcription initiation and promoter in LPTS/Pinx1 gene were also revealed. (C) 2004 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PUTATIVE-TUMOR-SUPPRESSOR ; HEPATOCELLULAR-CARCINOMA ; TELOMERASE ; MECHANISM ; CHROMATIN ; PINX1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220012800006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2062]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, SQ,Liao, C,Li, TP,et al. Cloning and characterization of the promoter region of human LPTS/PinX1 gene[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION,2004,1676(3):261-265. |
| APA | Wang, SQ,Liao, C,Li, TP,&Zhao, MJ.(2004).Cloning and characterization of the promoter region of human LPTS/PinX1 gene.BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION,1676(3),261-265. |
| MLA | Wang, SQ,et al."Cloning and characterization of the promoter region of human LPTS/PinX1 gene".BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 1676.3(2004):261-265. |
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