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Continuous zebularine treatment effectively sustains demethylation in human bladder cancer cells
文献类型：期刊论文
| 作者 | Cheng, JC; Weisenberger, DJ; Gonzales, FA; Liang, GN; Xu, GL; Hu, YG; Marquez, VE; Jones, PA |
| 刊名 | MOLECULAR AND CELLULAR BIOLOGY
 |
| 出版日期 | 2004 |
| 卷号 | 24期号:3页码:1270-1278 |
| 通讯作者 | Jones, PA (reprint author), Univ So Calif, Kenneth Norris Jr Comprehens Canc Ctr & Hosp, 1441 Eastlake Ave,Room 8302L,Mail Stop 83, Los Angeles, CA 90089 USA.,jones_p@ccnt.hsc.usc.edu |
| 英文摘要 | During tumorigenesis, tumor suppressor and cancer-related genes are commonly silenced by aberrant DNA methylation in their promoter regions. Recently, we reported that zebularine [1-(beta-D-ribofuranosyl)-1,2-dihydropyrimidin-2-one] acts as an inhibitor of DNA methylation and exhibits chemical stability and minimal cytotoxicity both in vitro and in vivo. Here we show that continuous application of zebularine to T24 cells induces and maintains p16 gene expression and sustains demethylation of the 5' region for over 40 days, preventing remethylation. In addition, continuous zebularine treatment effectively and globally demethylated various hypermethylated regions, especially CpG-poor regions. The drug caused a complete depletion of extractable DNA methyltransferase I (DNMT1) and partial depletion of DNMT3a and DNMT3b3. Last, sequential treatment with 5-aza-2'-deoxycytidine followed by zebularine hindered the remethylation of the p16 5' region and gene resilencing, suggesting the possible combination use of both drugs as a potential anticancer regimen. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | MESSENGER-RNA EXPRESSION ; DNA METHYLATION ; MYELODYSPLASTIC SYNDROMES ; CYTIDINE DEAMINASE ; CPG ISLANDS ; 5-AZA-2'-DEOXYCYTIDINE ; METHYLTRANSFERASES ; GENE ; INHIBITORS ; 5-AZACYTIDINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000188211500029 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2063]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Cheng, JC,Weisenberger, DJ,Gonzales, FA,et al. Continuous zebularine treatment effectively sustains demethylation in human bladder cancer cells[J]. MOLECULAR AND CELLULAR BIOLOGY,2004,24(3):1270-1278. |
| APA | Cheng, JC.,Weisenberger, DJ.,Gonzales, FA.,Liang, GN.,Xu, GL.,...&Jones, PA.(2004).Continuous zebularine treatment effectively sustains demethylation in human bladder cancer cells.MOLECULAR AND CELLULAR BIOLOGY,24(3),1270-1278. |
| MLA | Cheng, JC,et al."Continuous zebularine treatment effectively sustains demethylation in human bladder cancer cells".MOLECULAR AND CELLULAR BIOLOGY 24.3(2004):1270-1278. |
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