Toxicity of two type II ribosome-inactivating proteins (cinnamomin and ricin) to domestic silkworm larvae
文献类型：期刊论文
| 作者 | Wei, GQ; Liu, RS; Wang, QO; Liu, WY |
| 刊名 | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
 |
| 出版日期 | 2004 |
| 卷号 | 57期号:4页码:160-165 |
| 关键词 | cinnamomin cytotoxicity ricin RNA-glycosidose sarcin/ricin domain silkworm |
| 通讯作者 | Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,wyliu@sibs.ac.cn |
| 英文摘要 | Cinnamomin and ricin are two type II ribosome-inactivating proteins. They exhibited a different toxicity to domestic silkworm (Bombyx mori) larvae by oral feeding bioassay. The LC50 of ricin to the silkworm larvae at third instar was much lower than that of cinnamomin. When the isolated 80S ribosome from domestic silkworm pupae was treated separately with the reduced cinnamomin or the reduced ricin, a specific RNA fragment (R-fragment) was produced as characterized by 8 M urea-denatured polyacrylamide gel (3.5%) electrophoresis. The purified A-chains of both cinnamomin and ricin showed a slightly different RNA N-glycosidase activity to the domestic silkworm pupal ribosome. It was proposed that the difference of their toxicity to domestic silkworm larvae was not related to their A-chains but to the properties of their B-chains. It was also found that the vomit obtained from the midgut of domestic silkworm larvae could hydrolyze these two proteins apparently to a similar extent. (C) 2004 Wiley-Liss, Inc. |
| 学科主题 | Biochemistry & Molecular Biology; Entomology; Physiology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Entomology ; Physiology |
| 关键词[WOS] | PURIFICATION ; CAMPHORIN ; CHAINS ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000225556500002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2065]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wei, GQ,Liu, RS,Wang, QO,et al. Toxicity of two type II ribosome-inactivating proteins (cinnamomin and ricin) to domestic silkworm larvae[J]. ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,2004,57(4):160-165. |
| APA | Wei, GQ,Liu, RS,Wang, QO,&Liu, WY.(2004).Toxicity of two type II ribosome-inactivating proteins (cinnamomin and ricin) to domestic silkworm larvae.ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,57(4),160-165. |
| MLA | Wei, GQ,et al."Toxicity of two type II ribosome-inactivating proteins (cinnamomin and ricin) to domestic silkworm larvae".ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 57.4(2004):160-165. |
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