Characterization of function of three domains in dishevelled-1: DEP domain is responsible fair membrane translocation of dishevelled-1
文献类型：期刊论文
| 作者 | Pan, WJ; Pang, SZ; Huang, T; Guo, HY; Wu, DQ; Li, L |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2004 |
| 卷号 | 14期号:4页码:324-330 |
| 关键词 | Wnt frizzled-7 dishevelled DIX PDZ DEP membrane translocation signal transduction |
| 通讯作者 | Li, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol,Grad Sch, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,lli@sibs.ac.cn |
| 英文摘要 | Wnt signaling plays an important role in embryogenesis and tumorgenesis. Although the mechanism about how Wnts transduce their signaling from receptor frizzled (Fz) to cytosol has not been understood, dishevelled (Dvl) protein was considered as the intersection of Wnt signal traffic. In this study, we characterized the function of three domains (DIX, PDZ and DEP) of Dvl-1 in canonical Wnt signal transduction and Dvl-1 membrane translocation. It was found both DIX and DEP domain were sufficient to block Wnt-3a-induced LEF-l transcriptional activity and free cytosol beta-catenin accumulation; whereas PDZ domain and a functional mutant form of DEP domain (DEP-KM) had no effect on canonical Wnt signaling. In addition, when cotransfected with Fz-7, DEP domain, but not DIX, PDZ or DEP-KM, translocated and co-localized with Fz-7 to the plasma membrane, which was similar to Dvl-l. Furthermore, it was DEP domain that could block Fz-7-induced membrane translocation of Dvl-l via a possible competitive mechanism. These results strongly suggest that DEP domain is responsible for the membrane translocation of Dvl-l protein upon Wnt signal stimulation. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | WNT SIGNALING PATHWAY ; BETA-CATENIN ; FRIZZLED RECEPTORS ; PLANAR POLARITY ; PROTEINS ; MECHANISMS ; DROSOPHILA ; CANCER ; KINASE ; LEF-1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000223839200007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2068]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Pan, WJ,Pang, SZ,Huang, T,et al. Characterization of function of three domains in dishevelled-1: DEP domain is responsible fair membrane translocation of dishevelled-1[J]. CELL RESEARCH,2004,14(4):324-330. |
| APA | Pan, WJ,Pang, SZ,Huang, T,Guo, HY,Wu, DQ,&Li, L.(2004).Characterization of function of three domains in dishevelled-1: DEP domain is responsible fair membrane translocation of dishevelled-1.CELL RESEARCH,14(4),324-330. |
| MLA | Pan, WJ,et al."Characterization of function of three domains in dishevelled-1: DEP domain is responsible fair membrane translocation of dishevelled-1".CELL RESEARCH 14.4(2004):324-330. |
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