Production of recombinant human osteoprotegrin from Trichoplusia ni cells and Bombyx mori larvae
文献类型：期刊论文
| 作者 | Liu, Z; Yang, GZ; Wu, XF |
| 刊名 | PROTEIN AND PEPTIDE LETTERS
 |
| 出版日期 | 2004 |
| 卷号 | 11期号:4页码:317-323 |
| 关键词 | OPG mutant Trichophisia ni cell Bombyx mori larvae hypocalcemic effect |
| 通讯作者 | Wu, XF (reprint author), Chinese Acad Sci, Shanghai Inst BIol Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xfwu@sunm.shcnc.ac.cn |
| 英文摘要 | Human osteoprotegrin (OPG) and its truncated mutant OPG-280 and lengthened mutant OPG-Fc were constructed and successfully expressed in Trichophisia ni cells and Bombyx mori larvae. Native SDS-PAGE and Western blot analysis revealed that OPG-Fc is present as a homodimer in To cells or B. mori larvae compared with OPG and OPG-290. Furthermore, the hypocalcemic effect assay showed that truncation of the C-terminal 100 residues OPG does not abolish the biological activity and Fc can be helpful in forming the OPG homodimer with improved biological activity. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | OSTEOCLASTOGENESIS INHIBITORY FACTOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000222704000005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2071]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Z,Yang, GZ,Wu, XF. Production of recombinant human osteoprotegrin from Trichoplusia ni cells and Bombyx mori larvae[J]. PROTEIN AND PEPTIDE LETTERS,2004,11(4):317-323. |
| APA | Liu, Z,Yang, GZ,&Wu, XF.(2004).Production of recombinant human osteoprotegrin from Trichoplusia ni cells and Bombyx mori larvae.PROTEIN AND PEPTIDE LETTERS,11(4),317-323. |
| MLA | Liu, Z,et al."Production of recombinant human osteoprotegrin from Trichoplusia ni cells and Bombyx mori larvae".PROTEIN AND PEPTIDE LETTERS 11.4(2004):317-323. |
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