Chemically modified porcine hemoglobins and their biological properties
文献类型：期刊论文
| 作者 | Jin, C; Meng, WF; Min, H; Cen, PL; Yuan, ZY |
| 刊名 | PROTEIN AND PEPTIDE LETTERS
 |
| 出版日期 | 2004 |
| 卷号 | 11期号:4页码:353-360 |
| 关键词 | porcine hemoglobin (pHb) PEG DBBF modification immunogenicity resuscitation fluid |
| 通讯作者 | Jin, C (reprint author), Zhejiang Univ, Coll Mat & Chem Engn, Hangzhou 310027, Peoples R China., |
| 英文摘要 | Hemoglobin cross-linked with small molecular modifiers turns Out to be more stable. Modifications of proteins with polyethylene glycol (PEG) have been proven to enlarge the molecular size of proteins, to prolong their retention time in the circulation as well as blunt immune reactions. In the present study. the optimal conditions for porcine hemoglobin (pHb) modification with bis (3, 5-dibromosalicyl) fumarate (DBBF) and PEG were evaluated. The derivative of DBBF cross-linked pHb (DBBF-pHb) showed improved oxygen affinity and the ability to resist the dissociation of the alpha(2)beta(2) tetramer compared with the natural protein. DBBF-pHb was then bound to the activated PEG. The results indicated that the pHb modified with DBBF and PEG had more stable tetrameric conformation with a molecular weight of 107000. Their oxygen half-saturation pressure (P-50) is around 3.33 kPa, which approximates the physiological No or human red blood cells. Both routine and reinforced immunizing methods were adopted to study the immunogenicity of modified products and the results showed that the products had very low immunogenicity evaluated by enzyme-linked immunoadsordent assay (ELISA). Somewhat beneficial effects were shown in the treatment of hemorrhagic shock where modified hemoglobin solutions were used as resuscitation fluids in the hemorrhagic shock Sprague-Dawley (SD) rats model. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | POLYETHYLENE-GLYCOL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000222704000010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2072]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jin, C,Meng, WF,Min, H,et al. Chemically modified porcine hemoglobins and their biological properties[J]. PROTEIN AND PEPTIDE LETTERS,2004,11(4):353-360. |
| APA | Jin, C,Meng, WF,Min, H,Cen, PL,&Yuan, ZY.(2004).Chemically modified porcine hemoglobins and their biological properties.PROTEIN AND PEPTIDE LETTERS,11(4),353-360. |
| MLA | Jin, C,et al."Chemically modified porcine hemoglobins and their biological properties".PROTEIN AND PEPTIDE LETTERS 11.4(2004):353-360. |
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