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Exploration of pressure-induced dissociation of pyruvate oxidase

文献类型：期刊论文

作者Chang, TF; Ruan, KC
刊名CELLULAR AND MOLECULAR BIOLOGY
出版日期2004
卷号50期号:4页码:323-328
关键词pyruvate oxidase dissociation high pressure fluorescence spectroscopy
通讯作者Ruan, KC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Proteom, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,kcruan@sibs.ac.cn
英文摘要The dissociation of pyruvate oxidase (PO) caused by pressure up to 220 MPa at various conditions was explored by measuring the intrinsic fluorescence spectra and polarization. At 5degreesC and pH 7.6 the standard volume change (triangleV(0)) and free energy upon dissociation of the enzyme is -220 ml/mol and 29.83 kCal/mol, respectively. It was found that FAD was irreversibly removed during the pressure-dissociation of the enzyme. A much smaller standard volume change (-153 ml/mol) and lower free energy (24.92 kCal/mol) of apo-pyruvate oxidase (apo-PO) compared with the native enzyme indicated that FAD played very important role in stabilizing the enzyme and significantly influenced the standard volume change. The substrate pyruvic acid can significantly stabilize the enzyme against pressure in spite the standard volume for the enzyme in this case has a big increase relative to the native enzyme. The comparison of the intrinsic fluorescence of the native and the activated enzyme obtained by limited proteolysis indicated that the physical separation of a.-peptide from the enzyme only occurred when the subunits were dissociated from each other under pressure.
学科主题Biochemistry & Molecular Biology; Cell Biology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]HIGH HYDROSTATIC-PRESSURE ; CONFORMATIONAL DRIFT ; ESCHERICHIA-COLI ; PROTEASE ACTIVATION ; DEHYDROGENASE ; SITE ; BINDING
收录类别SCI
语种英语
WOS记录号WOS:000224691400004
版本出版稿
源URL[http://202.127.25.143/handle/331003/2073]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714		 Chang, TF,Ruan, KC. Exploration of pressure-induced dissociation of pyruvate oxidase[J]. CELLULAR AND MOLECULAR BIOLOGY,2004,50(4):323-328.
APA Chang, TF,&Ruan, KC.(2004).Exploration of pressure-induced dissociation of pyruvate oxidase.CELLULAR AND MOLECULAR BIOLOGY,50(4),323-328.
MLA Chang, TF,et al."Exploration of pressure-induced dissociation of pyruvate oxidase".CELLULAR AND MOLECULAR BIOLOGY 50.4(2004):323-328.

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来源：上海生物化学与细胞生物学研究所

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