Combination of targeting Gene-ViroTherapy with 5-FU enhances antitumor efficacy in malignant colorectal carcinoma
文献类型：期刊论文
| 作者 | Qiu, SB; Ruan, HM; Pei, ZF; Hu, BL; Lan, P; Wang, JH; Zhang, ZL; Gu, JF; Sun, LY; Qian, C |
| 刊名 | JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
 |
| 出版日期 | 2004 |
| 卷号 | 24期号:4页码:219-230 |
| 通讯作者 | Qi, YP (reprint author), Wuhan Univ, Coll Life Sci, Inst Virol, Wuhan 430072, Peoples R China.,qiyipeng@whu.edu.cn |
| 英文摘要 | To improve the therapeutic effect of ONYX015, an E1B55kD-deleted replication-competent adenovirus, ZD55 was constructed and armed with the therapeutic gene hTRAIL to form ZD55-hTRAIL, which was used for cancer therapy and which we call Targeting Gene-ViroTherapy. In vitro experiments with SW620, HCT116, and HT29 colorectal carcinoma cell lines demonstrated that they were all sensitive to ZD55-hTRAIL, and especially sensitive to ZD55-hTRAIL plus 5-fluorouracil (5-FU) treatment. In the SW620 xenograft tumor model, various treatment groups showed marked differences at week 11, with the tumor volume for the phosphate-buffered saline (PBS) treatment group >1700 mm(3), for 5-FU > 1300 mm(3), for ONYX015 1051.3 mm(3), for ZD55-hTRAIL 600.05 mm(3), and for ZD55-hTRAIL plus 5-FU 230.2 mm(3). At the end of week 14, tumor-bearing mice in the other groups almost all died, whereas all the mice in the combined treatment group were alive, with one mouse tumor free. By transmission electron microscopy (TEM) assay, most tumor cells treated with ONYX015 or with ZD55-hTRAIL singly or in combination with 5-FU were lysed due to viral propagation. RT-PCR analysis and immunohistochemistry examination revealed that hTRAIL was expressed in ZD55-hTRAIL-treated SW620 tumor tissue. Furthermore, no detectable hepatoxicity was found by serum enzyme level analysis. These results suggest that ZD55-hTRAIL alone or in combination with 5-FU may have potential clinical implications. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology; Immunology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology ; Immunology |
| 关键词[WOS] | APOPTOSIS-INDUCING LIGAND ; TUMOR-NECROSIS-FACTOR ; TRAIL-MEDIATED APOPTOSIS ; SELECTIVELY-REPLICATING ADENOVIRUS ; TUMORICIDAL ACTIVITY ; HUMAN HEPATOCYTES ; CANCER-CELLS ; DEATH DOMAIN ; NECK-CANCER ; IN-VIVO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000221057700002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2078]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Qiu, SB,Ruan, HM,Pei, ZF,et al. Combination of targeting Gene-ViroTherapy with 5-FU enhances antitumor efficacy in malignant colorectal carcinoma[J]. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH,2004,24(4):219-230. |
| APA | Qiu, SB.,Ruan, HM.,Pei, ZF.,Hu, BL.,Lan, P.,...&Qi, YP.(2004).Combination of targeting Gene-ViroTherapy with 5-FU enhances antitumor efficacy in malignant colorectal carcinoma.JOURNAL OF INTERFERON AND CYTOKINE RESEARCH,24(4),219-230. |
| MLA | Qiu, SB,et al."Combination of targeting Gene-ViroTherapy with 5-FU enhances antitumor efficacy in malignant colorectal carcinoma".JOURNAL OF INTERFERON AND CYTOKINE RESEARCH 24.4(2004):219-230. |
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