A trans-Golgi network retention signal YQRL fused to ricin A chain significantly enhances its cytotoxicity
文献类型：期刊论文
| 作者 | Zhan, JB; Ge, LP; Shen, JG; Wang, K; Zheng, S |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2004 |
| 卷号 | 313期号:4页码:1053-1057 |
| 关键词 | ricin A chain translocation trans-Golgi network retention signal |
| 通讯作者 | Zhan, JB (reprint author), Zhejiang Univ, Sch Med, Dept Biochem, 353 Yan An Rd, Hangzhou 310006, Peoples R China.,jzhan2k@cmm.zju.edu.cn |
| 英文摘要 | Ricin enters the cells by receptor-mediated endocytosis, followed by translocation across the membranes of intracellular organelles. A trans-Golgi retention peptide signal YQRL was fused to the C-terminus of ricin A chain (RTA) by polymerase chain reaction. The recombinant RTA and RTA-YQRL were expressed in Escherichia coli using plasmid pKK223.3 under the control of a tac promoter. The recombinant proteins were purified by affinity chromatography on a Blue-Sepharose 6B column. The cytotoxicities of RTA and the fusion toxin RTA-YQRL were measured by the MTT assay in HeLa, SKOV-3, and WISH cells following fluid-phase endocytosis. The rRTA-YQRL was 2-, 10-, and 40-fold more cytotoxic than rRTA itself in the three cell lines, respectively. The results indicate that addition of a TGN retention signal YQRL to the C-terminus of RTA can markedly increase its cytotoxicity, suggesting TGN may play an important role in the intracellular routing and translocation of RTA. (C) 2003 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PSEUDOMONAS EXOTOXIN ; TOXIN ; TRANSLOCATION ; SEQUENCE ; ANTIBODY ; CELLS ; KDEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000187997700034 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2085]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhan, JB,Ge, LP,Shen, JG,et al. A trans-Golgi network retention signal YQRL fused to ricin A chain significantly enhances its cytotoxicity[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,313(4):1053-1057. |
| APA | Zhan, JB,Ge, LP,Shen, JG,Wang, K,&Zheng, S.(2004).A trans-Golgi network retention signal YQRL fused to ricin A chain significantly enhances its cytotoxicity.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,313(4),1053-1057. |
| MLA | Zhan, JB,et al."A trans-Golgi network retention signal YQRL fused to ricin A chain significantly enhances its cytotoxicity".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 313.4(2004):1053-1057. |
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