Expression of overlapping PreS1 fragment recombinant proteins for the determination of immunogenic domains in HBsAg PreS1 region
文献类型：期刊论文
| 作者 | Hu, WG; Wei, J; Yang, XX; Xia, HC; Li, F; Zhang, ZC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2004 |
| 卷号 | 36期号:6页码:397-404 |
| 关键词 | HBV preS1 overlapping fragment immunogenic domain |
| 通讯作者 | Zhang, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Bio Sci, Inst Biochem & Cell Biol, Key Lab Proteom, Shanghai 200031, Peoples R China.,zhangzc@sunm.shcnc.ac.cn |
| 英文摘要 | In this study, eight preS I fragments overlapped in preS 1 (21-119) region of HBV adr subtype, i.e. preS1 (21-47), preS1 (34-59), preS1 (48-70), preS1 (60-85), preS1 (71-94), preS1 (86-109), preSI (95-119) and preS 1 (21-119), were cloned by PCR, and expressed as GST fusion proteins. These GST-preS I fusion proteins were highly expressed in soluble form in E. coli, and about 50 to 90 mg soluble fusion proteins were purified from I L culture. Using these fusion proteins, the immunogenic domains in preS 1 (2 1 119) region were identified by Western blot analysis and competitive ELISA. The results showed that the immunogenic domains mainly existed in preS 1 (21-59) in N-terminus and preS 1 (95-109) in C-terminus, and more importantly, a major immunogenic domain preS 1 (34-59), which has much stronger immunogenicity, was identified. It was also supported by the predictions of secondary structure and immunological property in the preS 1 (21-119) region. The results here would be helpful for the design of new vaccines against HBV |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | HEPATITIS-B-VIRUS ; RECEPTOR-BINDING SITE ; SURFACE-ANTIGEN ; T-CELL ; FINE SPECIFICITY ; EPITOPES ; PURIFICATION ; ANTIBODIES ; PEPTIDE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000222254900004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2098]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, WG,Wei, J,Yang, XX,et al. Expression of overlapping PreS1 fragment recombinant proteins for the determination of immunogenic domains in HBsAg PreS1 region[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(6):397-404. |
| APA | Hu, WG,Wei, J,Yang, XX,Xia, HC,Li, F,&Zhang, ZC.(2004).Expression of overlapping PreS1 fragment recombinant proteins for the determination of immunogenic domains in HBsAg PreS1 region.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(6),397-404. |
| MLA | Hu, WG,et al."Expression of overlapping PreS1 fragment recombinant proteins for the determination of immunogenic domains in HBsAg PreS1 region".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.6(2004):397-404. |
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