Nucleic acid binding activity of Pns6 encoded by genome segment 6 of rice ragged stunt oryzavirus
文献类型：期刊论文
| 作者 | Shao, CG; Lu, HJ; Wu, JH; Gong, ZX |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2004 |
| 卷号 | 36期号:7页码:457-466 |
| 关键词 | rice ragged stunt oryzavirus (RRSV) nucleic acid binding non-structural protein S6 |
| 通讯作者 | Gong, ZX (reprint author), Chinese Acad Sci, Shanghai Inst Life Sci, Key Lab Proteom, Inst Biochem & Cell Biol,Grad Sch, Shanghai 200031, Peoples R China.,gongzx@sunm.shcnc.ac.cn |
| 英文摘要 | The ORF of genome segment 6 (S6) of rice ragged stunt oryzavirus (RRSV) Philippines isolate was cloned and sequenced based on the S6 sequence of the Thailand isolate. Pns6, the 71 kD product of S6 expressed in E. coli, was demonstrated, to be a viral non-structural protein of RRSV by Western blotting. The gel mobility shift assays showed that Pns6 had nucleic acid binding activity. Pns6 could interact with single- and double-stranded forms of DNA and RNA, showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA, as demonstrated by both competition and displacement assays. The binding of Pif96 to nucleic acids is strong and sequence non-specific. By using five truncated derivatives of Pns6, it was found that the basic region from amino acid 201 to 273 of Pns6 was the unique nucleic acid binding domain. Subcellular fractionation of leaf tissues of RRSV-infected rice plants and subsequent Western blotting had shown that Pns6 accumulated predominately in the cytoplasmic membrane fraction. The possible role of RRSV Pns6 in virus replication and assembly is discussed. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | VIRUS-REPLICATION COMPLEXES ; TOBACCO MOSAIC-VIRUS ; MOVEMENT PROTEIN ; NONSTRUCTURAL PROTEINS ; ENDOPLASMIC-RETICULUM ; MEMBRANE ASSOCIATION ; STRUCTURAL PROTEIN ; RNA-POLYMERASE ; SPIKE PROTEIN ; STRANDED-RNA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000223224100003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2105]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Shao, CG,Lu, HJ,Wu, JH,et al. Nucleic acid binding activity of Pns6 encoded by genome segment 6 of rice ragged stunt oryzavirus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(7):457-466. |
| APA | Shao, CG,Lu, HJ,Wu, JH,&Gong, ZX.(2004).Nucleic acid binding activity of Pns6 encoded by genome segment 6 of rice ragged stunt oryzavirus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(7),457-466. |
| MLA | Shao, CG,et al."Nucleic acid binding activity of Pns6 encoded by genome segment 6 of rice ragged stunt oryzavirus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.7(2004):457-466. |
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