Antitumor effect of a novel adeno-associated virus vector targeting to telomerase activity in tumor cells
文献类型：期刊论文
| 作者 | Wang, YG; Wang, JH; Zhang, YH; Gu, Q; Liu, XY |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2004 |
| 卷号 | 36期号:7页码:492-500 |
| 关键词 | adeno-associated virus (AAV) telomerase hTERT promoter human interferon-beta cancer |
| 通讯作者 | Gu, Q (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,qinggu@21cn.com ; xyliu@sibs.ac.cn |
| 英文摘要 | Telomerase activity is a wide tumor marker. Human telomerase reverse transcriptase (hTERT), the catalytic subunit of the telomerase, is transcriptionally upregulated exclusively in about 90% of cancer cells. In this study, we constructed a novel adeno-associated virus (AAV) vector containing the human interferon-beta (hIFN-beta) gene under the control of hTERT promoter (AAV-hTERT-hIFN-beta) and investigated its antitumor effect against various human cancer cells in vitro. AAV-hTERT-hIFN-beta displayed cancer-specific hIFN-beta expression and cytotoxicity. The cytotoxic ratio was positively correlated with the time length of infection. AAV-hTERT-hIFN-beta-mediated apoptotic morphology was observed by transmission electron microscopy. Flow cytometry assay also revealed that the cytotoxicity of AAV-hTERT-hIFN-beta was mainly an apoptotic process. These data indicate that AAV in combination with hTERT-mediated therapeutic gene expression may open new possibilities for long-lasting and targeting gene therapy of varieties of cancers. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | CATALYTIC SUBUNIT HTERT ; EXPRESSING BACTERIAL NITROREDUCTASE ; REVERSE-TRANSCRIPTASE PROMOTER ; HUMAN CANCER-CELLS ; HUMAN BONE-MARROW ; GENE-THERAPY ; CELLULAR-TRANSFORMATION ; TRANSGENE EXPRESSION ; INDUCED APOPTOSIS ; BAX GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000223224100007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2106]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, YG,Wang, JH,Zhang, YH,et al. Antitumor effect of a novel adeno-associated virus vector targeting to telomerase activity in tumor cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(7):492-500. |
| APA | Wang, YG,Wang, JH,Zhang, YH,Gu, Q,&Liu, XY.(2004).Antitumor effect of a novel adeno-associated virus vector targeting to telomerase activity in tumor cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(7),492-500. |
| MLA | Wang, YG,et al."Antitumor effect of a novel adeno-associated virus vector targeting to telomerase activity in tumor cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.7(2004):492-500. |
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