Identification and characterization of a rat novel gene RSEP4 expressed specifically in central nervous system
文献类型：期刊论文
| 作者 | Wang, XD; Kong, LW; Xie, ZQ; Zhang, YQ; Lin, ZX; Zhao, ZQ; Yu, L; Jing, NH |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2004 |
| 卷号 | 36期号:7页码:501-507 |
| 关键词 | central nervous system (CNS) rat Northern blot in situ hybridization |
| 通讯作者 | Jing, NH (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Lab Mol Cell Biol, Shanghai 200031, Peoples R China.,njing@sibs.ac.cn |
| 英文摘要 | The low-abundantly expressed genes composed the majorities of the mRNAs expressed in the central nervous system (CNS), and were thought to be important for the normal brain functions. Through differential screening a low-abundance cDNA sublibrary with mRNA from neuropathic pain of chronic constriction injury (CCI) model, we have identified a novel rat gene, rat spinal-cord expression protein 4 gene (RSEP4). The total length of RSEP4 cDNA is 2006 bp, with a 501 nucleotide open reading frame (ORF) that encodes a 167 amino acid polypeptide. Northern blot revealed that RSEP4 was expressed specifically in the CNS. In situ hybridization showed that the mRNA of RSEP4 was strongly expressed in the CA1, CA2, CA3 and DG regions of hippocampus, the Purkinje cells of cerebellum, and the small sensory neurons of dorsal horn and large motor neurons of ventral horn of spinal cord. Over-expression of RSEP4-EGFP fusion protein in the human embryonic kidney 293T cells showed that RSEP4 protein was mainly localized in the cell cytoplasm. These results suggest that RSEP4 may play some roles in the CNS. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | NEUROPATHIC PAIN ; HIPPOCAMPUS ; ACTIVATION ; ALLODYNIA ; PET |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000223224100008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2107]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, XD,Kong, LW,Xie, ZQ,et al. Identification and characterization of a rat novel gene RSEP4 expressed specifically in central nervous system[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(7):501-507. |
| APA | Wang, XD.,Kong, LW.,Xie, ZQ.,Zhang, YQ.,Lin, ZX.,...&Jing, NH.(2004).Identification and characterization of a rat novel gene RSEP4 expressed specifically in central nervous system.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(7),501-507. |
| MLA | Wang, XD,et al."Identification and characterization of a rat novel gene RSEP4 expressed specifically in central nervous system".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.7(2004):501-507. |
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