Effect of voluntary exercise on genetically obese Cpe(fat/fat) mice: quantitative proteomics of serum
文献类型：期刊论文
| 作者 | Yuan, Q; Fontenele-Neto, JD; Fricker, LD |
| 刊名 | OBESITY RESEARCH
 |
| 出版日期 | 2004 |
| 卷号 | 12期号:7页码:1179-1188 |
| 关键词 | peptide processing carboxypeptidase E neuropeptide kallikrein haptoglobin |
| 通讯作者 | Fricker, LD (reprint author), Yeshiva Univ Albert Einstein Coll Med, Dept Mol Pharmacol, 1300 Morris Pk Ave, Bronx, NY 10461 USA.,fricker@aecom.yu.edu |
| 英文摘要 | Objective: To compare the effect of voluntary exercise on body weight, food consumption, and levels of serum proteins between wild-type and carboxypeptidase E-deficient (Cpe(fat/fat)) mice. Research Methods and Procedures: Study I consisted of three groups of female mice: Cpe(fat/f`at) mice with continuous access to exercise wheels for 3 weeks (n = 4); wild-type C57BKS mice with access to exercise wheels for 3 weeks (n = 4); and sedentary Cpe(fat/fat) mice (n = 3). Activity, body weight, and food consumption were monitored for this period and a subsequent 9-week period without exercise wheels. Study 2 consisted of four groups of male mice (n = 6 to 7 each): Cpe(fat)/(fat) mice with exercise wheels, wild-type mice with exercise wheels, and CPe(fat/fat) and wild-type mice without exercise wheels. Body weight and food consumption were measured over 4 weeks. Sera were collected, and the protein profile was determined by 2-dimensional gel electrophoresis and mass spectrometry. Results: Cpe(fat/fat) mice were moderately hyperphagic but lost weight during the initial exercise period because of greater energy expenditure. The effect of exercise was temporary, and the mice gained weight after the second week. Several serum proteins were found to be altered by exercise: haptoglobin was decreased by exercise in CPe(fat/fat) mice, and several kallikreins were increased by exercise in wildtype mice. Discussion: The access to exercise wheels provided an initial weight loss in Cpe(fat/fat) mice, but this effect was offset by elevated food consumption. The serum proteomics results indicated that Cpe(fat/fat) and wild-type mice differed in their response to exercise. |
| 学科主题 | Endocrinology & Metabolism; Nutrition & Dietetics |
| 类目[WOS] | Endocrinology & Metabolism ; Nutrition & Dietetics |
| 关键词[WOS] | FAT MUTATION ; BODY-WEIGHT ; FOOD-INTAKE ; PLASMA-PROTEINS ; GENE-EXPRESSION ; RATS ; ELECTROPHORESIS ; INFLAMMATION ; HAPTOGLOBIN ; KALLIKREIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000223158300018 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2110]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yuan, Q,Fontenele-Neto, JD,Fricker, LD. Effect of voluntary exercise on genetically obese Cpe(fat/fat) mice: quantitative proteomics of serum[J]. OBESITY RESEARCH,2004,12(7):1179-1188. |
| APA | Yuan, Q,Fontenele-Neto, JD,&Fricker, LD.(2004).Effect of voluntary exercise on genetically obese Cpe(fat/fat) mice: quantitative proteomics of serum.OBESITY RESEARCH,12(7),1179-1188. |
| MLA | Yuan, Q,et al."Effect of voluntary exercise on genetically obese Cpe(fat/fat) mice: quantitative proteomics of serum".OBESITY RESEARCH 12.7(2004):1179-1188. |
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