Studies on lectin activity of two type II ribosome-inactivating proteins isolated from mature seed of camphor tree
文献类型：期刊论文
| 作者 | Yang, HY; Li, RX; Liu, WY |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2004 |
| 卷号 | 31期号:7页码:650-654 |
| 关键词 | cinnamomin cinphorin lectin fluorescence spectrometry |
| 通讯作者 | Li, RX (reprint author), Chinese Acad Sci, Shanghai Inst Biol, State key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,rxli@sjtu.edu.cn ; wyliu@sibs.ac.cn |
| 英文摘要 | Two new type H ribosome-inactivating proteins ( RIPs) were isolated from the mature seed of camphor tree (Cinnamomum camphora). They are named cinnamomin and cinphorin. The molecular mass of cinphorin A-chain is only half of cinnamomin A-chain, while their B-chains are the same. Their A-chains are RNA N-glycosidases, and the B-chains are lectins. The intrinsic cytotoxicity of type H RIP is greatly dependent on the carbohydrate-binding activity and the specificity of its B-chain. The lectin activity of cinnamomin and cinphorin are investigated and compared with each other. They showed similar hemagglutination activity. Their saccharide binding specificities were studied by hapten inhibition, indicating that they were both galactose-specific. However, N-acetylgalactosamine failed to bind to the two RIPs as ricin/abrin did. The interactions of the two RIPs with specific saccharides were also investigated by fluorescence spectroscopy through which association constants were obtained. Their association constants of galactose or lactose were found to be identical. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | SACCHARIDE BINDING ; CINNAMOMIN |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000222914400013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2112]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, HY,Li, RX,Liu, WY. Studies on lectin activity of two type II ribosome-inactivating proteins isolated from mature seed of camphor tree[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(7):650-654. |
| APA | Yang, HY,Li, RX,&Liu, WY.(2004).Studies on lectin activity of two type II ribosome-inactivating proteins isolated from mature seed of camphor tree.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,31(7),650-654. |
| MLA | Yang, HY,et al."Studies on lectin activity of two type II ribosome-inactivating proteins isolated from mature seed of camphor tree".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 31.7(2004):650-654. |
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