Monoclonal antibodies assisting refolding of firefly luciferase
文献类型：期刊论文
| 作者 | Xu, Q; Xie, ZQ; Ding, JF; Lin, SX; Xu, GJ |
| 刊名 | PROTEIN SCIENCE
 |
| 出版日期 | 2004 |
| 卷号 | 13期号:7页码:1851-1858 |
| 关键词 | luciferase protein unfolding and refolding protein aggregation monoclonal antibodies epitopes enzyme activity |
| 通讯作者 | Xu, GJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,gjxu@sibs.ac.cn |
| 英文摘要 | The reactivation efficiency in the refolding of denatured luciferase in the presence and the absence of monoclonal antibodies (mAbs) has been studied. Luciferase could be partially reactivated when the protein was denatured in high concentrations of guanidium chloride (GdmCl; >4.5 M) and the refolding was carried out in very low protein concentrations. The refolding yield was, however, significantly lower when it was performed on luciferase that had been denatured with lower concentrations of GdmCl. The efficiency of refolding decreases when the formation of aggregates increases. Three of the five luciferase mAbs tested (4G3, N20, S2G10) dramatically increased the yield of reactivation and simultaneously eliminated the formation of aggregates. It is proposed that these mAbs assisted the refolding of luciferase by binding to the exposed hydrophobic surface of the refolding intermediate, thus preventing it from aggregating. The epitopes interacting with these refolding-assisting mAbs are all located in the A-subdomain of the N-terminal region of luciferase. These results have also shed light on the structural features of the intermediate and its interface involved in protein aggregate formation, contributing to the understanding of the protein folding mechanism. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | PROTEIN ; AGGREGATION ; LYSOZYME ; CHAINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000222283300013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2113]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, Q,Xie, ZQ,Ding, JF,et al. Monoclonal antibodies assisting refolding of firefly luciferase[J]. PROTEIN SCIENCE,2004,13(7):1851-1858. |
| APA | Xu, Q,Xie, ZQ,Ding, JF,Lin, SX,&Xu, GJ.(2004).Monoclonal antibodies assisting refolding of firefly luciferase.PROTEIN SCIENCE,13(7),1851-1858. |
| MLA | Xu, Q,et al."Monoclonal antibodies assisting refolding of firefly luciferase".PROTEIN SCIENCE 13.7(2004):1851-1858. |
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