An effective method for raising antisera against beta-defensins: Double-copy protein expression of mBin1b in E-coli
文献类型：期刊论文
| 作者 | Xiao, LQ; Liu, AH; Zhang, YL |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2004 |
| 卷号 | 36期号:8页码:571-576 |
| 关键词 | Bin1b epididymis beta-defensin double-copy protein polyclonal antiserum |
| 通讯作者 | Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol,Grad Sch, Shanghai 200031, Peoples R China.,ylzhang@sibs.ac.cn |
| 英文摘要 | Bin1b is a rat epididymis specific beta-defensin which may have fertility related functions in addition to its antimicrobial activity. beta-defensins are cysteine-rich cationic antimicrobial peptides that have their important implications in innate and adaptive immunity. Though considerable numbers of new beta-defensins have been discovered, few corresponding antibodies have been reported. The small peptide with special structure and antimicrobial nature of beta-defensins make them very difficult to express in prokaryotic system. Here we adopted a double-copy protein expression scheme based on which not only the mBin1b protein was successfully expressed but also the immunity of the antigen was enhanced. The validity of the antisera was verified by using Western blotting and immunohistochemical analyses. It will be a useful toot for deeply investigating the roles of Bin1b and also provide a simple but effective method in raising antisera against other members of the beta-defensin gene family. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ANTIMICROBIAL PEPTIDE ; HUMAN BETA-DEFENSIN-2 ; POLYCLONAL ANTISERA ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000223682000009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2114]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xiao, LQ,Liu, AH,Zhang, YL. An effective method for raising antisera against beta-defensins: Double-copy protein expression of mBin1b in E-coli[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(8):571-576. |
| APA | Xiao, LQ,Liu, AH,&Zhang, YL.(2004).An effective method for raising antisera against beta-defensins: Double-copy protein expression of mBin1b in E-coli.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(8),571-576. |
| MLA | Xiao, LQ,et al."An effective method for raising antisera against beta-defensins: Double-copy protein expression of mBin1b in E-coli".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.8(2004):571-576. |
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