Overexpression of acetylcholinesterase inhibited cell proliferation and promoted apoptosis in NRK cells
文献类型：期刊论文
| 作者 | Jin, QH; He, HY; Shi, YF; Lu, H; Zhang, XJ |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2004 |
| 卷号 | 25期号:8页码:1013-1021 |
| 关键词 | acetylcholinesterase gene expression proliferation apoptosis |
| 通讯作者 | Zhang, XJ (reprint author), Chinese Acad Sci, Grad Sch, Chinese Acad Sci, Mol Cell Biol Lab,Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,xjzhang@sibs.ac.en |
| 英文摘要 | AIM: To study the potential function of acetylcholinesterase (AChE) in apoptosis through overexpression of AChE in Normal Rat Kidney (NRK) cells. METHODS: AChE activity was detected by the method of Karnovsky and Roots. Activated caspase-3 was analyzed by Western blotting and immunoflurescence with antibody special to activated caspase-3 fragment. The expression plasmids were constructed in pcDNA3.1 containing AChE gene or a fragment of AChE antisense that were got from RT-PCR. Stable expression cell lines were selected by G418 in cells transfected by lipofection. AChE expression was analyzed by RT-PCR and Western blotting. The proliferation rates of transfected cells were examined by the growth curve and cloning efficiency. MTT assay was used to analyze the cell viability. RESULTS: The proliferation rate of the cells transfected with AChE was retarded and the cloning efficiency was lower (28.2 % +/-3.1 % and 48.7 % +/-2.1 %) than cells transfected with vector (56.1 % +/-0.3 %) or AChE-antisense (77.7 %+/-2.2 %). After 2 d the various clone types were deprived of serum, the residue cell viability were 10.4 %+/-4.6 % and 12.6 %+/-6.7 % in the cells transfected with ACK, and 27.4 %+/-3.5 % in cells with vector, and 50.3 %+/-7.8 % in cells with AChE-antisense. CONCLUSION: During apoptosis, increase of AChE protein is to inhibit cell proliferation, and then to promote apoptosis in NRK cells. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | GENE-EXPRESSION ; MOLECULAR-FORMS ; INCREASES ; DIFFERENTIATION ; INDUCTION ; ENZYME ; BRAIN ; VIVO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000223240900007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2115]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jin, QH,He, HY,Shi, YF,et al. Overexpression of acetylcholinesterase inhibited cell proliferation and promoted apoptosis in NRK cells[J]. ACTA PHARMACOLOGICA SINICA,2004,25(8):1013-1021. |
| APA | Jin, QH,He, HY,Shi, YF,Lu, H,&Zhang, XJ.(2004).Overexpression of acetylcholinesterase inhibited cell proliferation and promoted apoptosis in NRK cells.ACTA PHARMACOLOGICA SINICA,25(8),1013-1021. |
| MLA | Jin, QH,et al."Overexpression of acetylcholinesterase inhibited cell proliferation and promoted apoptosis in NRK cells".ACTA PHARMACOLOGICA SINICA 25.8(2004):1013-1021. |
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