Role of domains in factor C from Tachypleus tridentatus in neutralization endotoxin
文献类型：期刊论文
| 作者 | Sun, XJ; Wang, DN; Zhang, WJ; Wu, XF |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2004 |
| 卷号 | 31期号:8页码:736-740 |
| 关键词 | LPS-neutralization LPS-binding activity LPS-binding site bactericidal activity in vitro |
| 通讯作者 | Zhang, WJ (reprint author), Shanghai Jiao Tong Univ, Coll Life Sci & Technol, Shanghai 200030, Peoples R China.,yrszhang@hotmail.com ; xfwu@sonar.shenc.ac.cn |
| 英文摘要 | Factor C is a serine protease enzymogen presented in the circulating blood cell of horseshoe crab. Owing to its extreme sensitivity to endotoxin, Factor C (FC) plays an important role in pyrogen-detection in pharmaceutical products. Previous study has shown that three Sushi domains (S123) in the N-terminal of FC are critical for its LPS-recognition activity. The effect of three other domains in N-terminal of FC, namely Cys-rich, EGF-like and Lectin-like, on its LPS-neutralization function was not clear. The recombinant FC and its four truncated fragments were expressed using Bac-to-Bac baculovirus expression system in Tn cells. The five recombinant peptides were then purified and tested for the LPS-binding activity and bactericidal activity in vitro. The experiments showed that the LPS-binding site of Factor C resides in the S123 region. Even though Cys-rich, EGF-like and Lectin-like domains do not harbor LPS-binding site, the presence of all three domains can improve LPS-binding activity of S123. Recombinant peptide rCES123L containing Cys-rich, EGF-like, S123, and Lectin-like domains exhibits almost the same LPS-binding activity as that of the full-length parental FC. Provided the fact that rCES123L has 4-fold higher production yield than that of recombinant FC in Tn cells, this peptide has a potential in biotechnology and pharmaceutical application for LPS-neutralization. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | SERINE-PROTEASE ZYMOGEN ; RECOMBINANT FACTOR C ; ANTI-LPS FACTOR ; LIPOPOLYSACCHARIDE ; HEMOCYTES |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000223647600013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2120]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Sun, XJ,Wang, DN,Zhang, WJ,et al. Role of domains in factor C from Tachypleus tridentatus in neutralization endotoxin[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(8):736-740. |
| APA | Sun, XJ,Wang, DN,Zhang, WJ,&Wu, XF.(2004).Role of domains in factor C from Tachypleus tridentatus in neutralization endotoxin.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,31(8),736-740. |
| MLA | Sun, XJ,et al."Role of domains in factor C from Tachypleus tridentatus in neutralization endotoxin".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 31.8(2004):736-740. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。