A novel scorpion toxin blocking small conductance Ca2+ activated K+ channel
文献类型：期刊论文
| 作者 | Xu, CQ; He, LL; Brone, B; Martin-Eauclaire, MF; Van Kerkhove, E; Zhou, Z; Chi, CW |
| 刊名 | TOXICON
 |
| 出版日期 | 2004 |
| 卷号 | 43期号:8页码:961-971 |
| 关键词 | SK channel amino acid sequence gene cloning phylogenetic tree |
| 通讯作者 | Chi, CW (reprint author), Chinese Acad Sci, Key Lab Proteom, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,chi@sunm.shcnc.ac.cn |
| 英文摘要 | Small conductance calcium activated potassium channels (SK) are crucial in the regulation of cell firing frequency in the nervous system and other tissues. In the present work, a novel SK channel blocker, designated BmSKTx1, was purified from the scorpion Buthus martensi Karsh venom. The sequence of the N-terminal 22 amino acid residues was determined by Edman degradation. Using this sequence information, the full-length cDNA and genomic gene of BmSKTx1 were cloned and sequenced. By these analyses, BmSKTx1 was found to be a peptide composed of 31 amino acid residues with three disulfide bonds. It shared little sequence homology with other known scorpion alpha-KTxs but showed close relationship with SK channel blockers in the phylogenetic tree. According to the previous nomenclature, BmSKTx1 was classified as alpha-KTx14.1. We examined the effects of BmSKTx1 on different ion channels of rat adrenal chromaffin cells (RACC) and locust dorsal unpaired median (DUM) neurons. BmSKTx1 selectively inhibited apamin-sensitive SK currents in RACC with K-d of 0.72 p,M and Hill coefficient of 2.2. And it had no effect on Na+, Ca2+, Kv, and BK currents in DUM neuron, indicating that BmSKTx1 was a selective SK toxin. (C) 2004 Elsevier Ltd. All rights reserved. |
| 学科主题 | Pharmacology & Pharmacy; Toxicology |
| 类目[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 关键词[WOS] | ADRENAL CHROMAFFIN CELLS ; BUTHUS-MARTENSI KARSCH ; UNPAIRED MEDIAN NEURONS ; POTASSIUM CHANNELS ; GATING MODIFIER ; LEIUROTOXIN-I ; ION CHANNELS ; CALCIUM ; VENOM ; APAMIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000222564500012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2121]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, CQ,He, LL,Brone, B,et al. A novel scorpion toxin blocking small conductance Ca2+ activated K+ channel[J]. TOXICON,2004,43(8):961-971. |
| APA | Xu, CQ.,He, LL.,Brone, B.,Martin-Eauclaire, MF.,Van Kerkhove, E.,...&Chi, CW.(2004).A novel scorpion toxin blocking small conductance Ca2+ activated K+ channel.TOXICON,43(8),961-971. |
| MLA | Xu, CQ,et al."A novel scorpion toxin blocking small conductance Ca2+ activated K+ channel".TOXICON 43.8(2004):961-971. |
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