The role of propeptide in the refolding of human group IB phospholipase A(2)
文献类型：期刊论文
| 作者 | Cheng, HQ; Xu, GJ |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2004 |
| 卷号 | 36期号:9页码:583-588 |
| 关键词 | human group IB phospholipase A(2) (IB-PLA(2)) refolding first disulfide bond propeptide |
| 通讯作者 | Xu, GJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,gjxu@sibs.ac.cn |
| 英文摘要 | Human group IB phospholipase A(2) (IB-PLA(2)) and its zymogen (proIB-PLA(2)) were purified from E. colt. Refolding was carried out by diluting the denatured forms of both IB-PLA(2) and proIB-PLA(2) with renaturation buffer in which the disulfide bonds were completely reduced. The refolding yield of proIB-PLA(2) was increased by about 50% over that of the mature enzyme. The refolding of IB-PLA(2) usually produced aggregates under normal conditions, as determined by light scattering. In addition, the unfolding experiments showed that the mature enzyme was more stable than the proenzyme toward denaturants in the presence of DTT. Results suggested that the N-terminal sequence rather than its conformation of human prolB-PLA(2) played an important role in the refolding process. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PRO-REGION ; CHAPERONE ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000223987100001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2124]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Cheng, HQ,Xu, GJ. The role of propeptide in the refolding of human group IB phospholipase A(2)[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(9):583-588. |
| APA | Cheng, HQ,&Xu, GJ.(2004).The role of propeptide in the refolding of human group IB phospholipase A(2).ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(9),583-588. |
| MLA | Cheng, HQ,et al."The role of propeptide in the refolding of human group IB phospholipase A(2)".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.9(2004):583-588. |
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