Detection of tumor marker CA125 in ovarian carcinoma using quantum dots
文献类型：期刊论文
| 作者 | Wang, HZ; Wang, HY; Liang, RQ; Ruan, KC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2004 |
| 卷号 | 36期号:10页码:681-686 |
| 关键词 | quantum dots detection of tumor marker CA125 ovarian carcinoma |
| 通讯作者 | Ruan, KC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom,Grad Sch, Shanghai 200031, Peoples R China.,kcruan@sibs.ac.cn |
| 英文摘要 | Semiconductor quantum dots (QDs) offer several advantages over organic dyes in fluorescence-imaging applications, such as higher quantum yield, exceptional photostability, and a narrow, tunable, and symmetric emission spectrum. To explore whether QDs could specifically and effectively label tumor markers and be used in immunohistochemistry as a novel type of fluorescent probe, we used quantum dots with maximum emission wavelength 605 nm (QD605) to detect the ovarian carcinoma marker CA125 in specimens of different types (fixed cells, tissue sections, and xenograft piece). Additionally, we compared the photostability of QD signals with that of a conventional organic dye, FITC. All labeling signals of QDs were found to be more specific and brighter than those of FITC. Moreover, the QDs exhibited exceptional photostability during continuous illumination for 1 h by a high-intensity laser (Ar laser power 100 mW) at 488 nm, while the FITC signals faded very quickly and became undetectable after 24 min of illumination. These results indicate that QD-based probes can offer substantial advantages over existing fluorophores in many applications, and can be used effectively in immunohistochemistry as a novel class of fluorescent probes. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | SEMICONDUCTOR NANOCRYSTALS ; IN-VIVO ; CELLS ; LABELS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000224758500006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2134]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, HZ,Wang, HY,Liang, RQ,et al. Detection of tumor marker CA125 in ovarian carcinoma using quantum dots[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(10):681-686. |
| APA | Wang, HZ,Wang, HY,Liang, RQ,&Ruan, KC.(2004).Detection of tumor marker CA125 in ovarian carcinoma using quantum dots.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(10),681-686. |
| MLA | Wang, HZ,et al."Detection of tumor marker CA125 in ovarian carcinoma using quantum dots".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.10(2004):681-686. |
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