LFIRE-1/HFREP-1, a liver-specific gene, is frequently downregulated and has growth suppressor activity in hepatocellular carcinoma
文献类型：期刊论文
| 作者 | Yan, J; Yu, YL; Wang, N; Chang, YC; Ying, H; Liu, WZ; He, J; Li, SQ; Jiang, WL; Li, YL |
| 刊名 | ONCOGENE
 |
| 出版日期 | 2004 |
| 卷号 | 23期号:10页码:1939-1949 |
| 关键词 | hepatocellular carcinoma (HCC) 8p22 fibrinogen-related domain liver-specific expression differentiation |
| 通讯作者 | Xu, YH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol & Cellular Oncol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,yhxu@sunm.shcnc.ac.cn |
| 英文摘要 | We have previously identified several novel genes, which are differentially expressed among human normal liver and hepatocellular carcinomas (HCCs). The full-length liver fibrinogen-related gene-1 (LFIRE-1) cDNA was cloned from the human normal liver cDNA library. LFIRE-1 is highly homologous to HFREP-1 with discrepancy at 50 untranslated region (UTR) and encodes the same fibrinogen-related protein, which suggest that these two sequences might be alternative splicing forms of the same gene, LFIRE-1/HFREP-1, located at human chromosome 8p22. The LFIRE-1 and HFREP-1 are specifically expressed in normal human liver tissue, but reduced or undetectable in most of HCC specimens at both RNA and protein level. Furthermore, the reduction or nonexpression of LFIRE-1/HFREP-1 is significantly associated with the degree of tumor differentiation. Loss of heterozygosity (LOH) analysis revealed allelic loss of LFIRE-1/HFREP-1 on chromosome 8p22 in 57.1% (24/42) of HCC specimens. We detected three inactivation mutations among 45 cases of HCC specimens examined, two of which lost the remaining allele and the third had a replacement of conserved cysteine residue with glycine residue. Notably, the downregulation of LFIRE-1/HFREP-1 expression is frequently associated with allelic loss. The reduction of LFIRE-1/HFREP-1 expression by antisense approach enhances cancer cell proliferation and colony formation in soft agar. Moreover, restoration of exogenous wild-type LFIRE-1/HFREP-1 expression but not LFIRE-1/HFREP-1 missense mutations in human HCC cells inhibited their anchorage-dependent or independent growth in vitro, and suppressed their tumorigenicity in nude mice. In conclusion, our data demonstrated that liver-specific gene LFIRE-1/HFREP-1 was frequently downregulated and might possess growth suppressor activity in HCC. |
| 学科主题 | Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity |
| 类目[WOS] | Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity |
| 关键词[WOS] | CANCER EPIGENETICS ; CHROMOSOME 8P ; CLONING ; PROGRESSION ; STAGE ; ARM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220129500016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2151]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yan, J,Yu, YL,Wang, N,et al. LFIRE-1/HFREP-1, a liver-specific gene, is frequently downregulated and has growth suppressor activity in hepatocellular carcinoma[J]. ONCOGENE,2004,23(10):1939-1949. |
| APA | Yan, J.,Yu, YL.,Wang, N.,Chang, YC.,Ying, H.,...&Xu, YH.(2004).LFIRE-1/HFREP-1, a liver-specific gene, is frequently downregulated and has growth suppressor activity in hepatocellular carcinoma.ONCOGENE,23(10),1939-1949. |
| MLA | Yan, J,et al."LFIRE-1/HFREP-1, a liver-specific gene, is frequently downregulated and has growth suppressor activity in hepatocellular carcinoma".ONCOGENE 23.10(2004):1939-1949. |
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