Expression, purification and sublocalization of SARS-CoV nucleocapsid protein in insect cells
文献类型：期刊论文
| 作者 | Ren, AX; Xie, YH; Kong, YY; Yang, GZ; Zhang, YZ; Wang, Y; Wu, XF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2004 |
| 卷号 | 36期号:11页码:754-758 |
| 关键词 | severe acute respiratory syndrome (SARS) SARS coronavirus nucleocapsid protein baculovirus insect cells |
| 通讯作者 | Wang, Y (reprint author), Zhejiang Univ, Inst Biochem & Biotechnol, Coll Life Sci, Hangzhou 310029, Peoples R China.,yaozhou@chinagene.com |
| 英文摘要 | The causative agent of severe acute respiratory syndrome (SARS) is a previously unidentified coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is a major viral protein recognized by acute and early convalescent sera from SARS patients. To facilitate the studies on the function and structure of the N protein, this report describe the expression and purification of recombinant SARS-CoV N protein using the baculovirus expression system. Recombinant hexa-histidine-tagged N protein with a molecular mass of 47 kD was produced in insect cells. Recombinant N protein was purified to near homogeneity by Ni2+-NTA affinity chromatography. In addition, we examined the subcellular localization of the N protein by confocal microscopy in Trichoplusia ni BT1 Tn 5B1-4 cells infected with recombinant baculovirus. The N protein was found localized in the cytoplasm as well as in the nucleolus. The purified recombinant N protein can be used in further functional study of SARS-CoV. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ACUTE RESPIRATORY SYNDROME ; HOST-CELL ; CORONAVIRUS ; ANTIBODIES ; NUCLEOPROTEIN ; NUCLEOLUS ; DIAGNOSIS ; IDENTIFICATION ; LOCALIZATION ; SEQUENCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000225325300006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2154]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ren, AX,Xie, YH,Kong, YY,et al. Expression, purification and sublocalization of SARS-CoV nucleocapsid protein in insect cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(11):754-758. |
| APA | Ren, AX.,Xie, YH.,Kong, YY.,Yang, GZ.,Zhang, YZ.,...&Wu, XF.(2004).Expression, purification and sublocalization of SARS-CoV nucleocapsid protein in insect cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(11),754-758. |
| MLA | Ren, AX,et al."Expression, purification and sublocalization of SARS-CoV nucleocapsid protein in insect cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.11(2004):754-758. |
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