Study on Bombyx mori nuclear polyhedrosis virus (BmNPV) ubiquitin and its binding peptides
文献类型：期刊论文
| 作者 | Zhang, WB; Lin, R; Zhang, YZ; Wu, XF |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2004 |
| 卷号 | 31期号:11页码:1038-1044 |
| 关键词 | BmNPV ubiquitin phage display peptide library binding peptide |
| 通讯作者 | Zhang, YZ (reprint author), Zhejiang Univ Sci, Inst Biochem, Hangzhou 310018, Peoples R China.,yaozhou@chinagene.com |
| 英文摘要 | The ubiquitin (UB) gene of Bombyx mori nuclear polyhedrosis virus Zhenjiang strain (BmNPV-ZJ) was cloned and sequenced. Sequencing analysis shows that the BmVUB gene, which contains 234 nucleotides encoding 77 amino acids, contains a conservative HTH, a conservative LRLRGG and four conservative functional sites: Lys-29, Cys-48, Cys-63, Gly-76 which play important roles in ubiquitin-proteasome complex formation. It also shows that the C-terminal amino acid was redundant which locate at the downstream of conservative Gly-Gly proteinase cleavage signal sequence which characterizes as Gly-Gly-X ( X was hydrophobic amino acid). The BmVUB gene was induced and highly expressed in E. colt BL21. The results of gel scanning and Bradford protein assay show that the BmVUB protein was about 9 ku and the yield consists of 50 percent of total bacterial protein. The concentration of purified BmVUB protein was 1.03 similar to 2.46 g/L. The high titer antibody wag obtained from rabbits immunized with the (His)(6) - BmVUB fusion protein and ELISA shows that the titer of antibody was over 3.2 X 10(-5). Peptides binding ubiquitin were obtained from phage displayed peptide library using BmNPV-ZJ ubiquitin purified with His-affinity chromatography as a ligand. Five short peptides were identified which displayed obvious biological activity. Peptide VAPHHAYAPMRT could regulate the cell proliferation and further more this function which depends on the concentration variation, namely low concentration could promote the cell proliferation, whereas high concentration will inhibit the proliferation. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | APOPTOSIS ; GENE ; MDM2 |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000225377500014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2155]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, WB,Lin, R,Zhang, YZ,et al. Study on Bombyx mori nuclear polyhedrosis virus (BmNPV) ubiquitin and its binding peptides[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(11):1038-1044. |
| APA | Zhang, WB,Lin, R,Zhang, YZ,&Wu, XF.(2004).Study on Bombyx mori nuclear polyhedrosis virus (BmNPV) ubiquitin and its binding peptides.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,31(11),1038-1044. |
| MLA | Zhang, WB,et al."Study on Bombyx mori nuclear polyhedrosis virus (BmNPV) ubiquitin and its binding peptides".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 31.11(2004):1038-1044. |
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