Solution structure of BmBKTx1, a new BKCa1 channel blocker from the Chinese scorpion Buthus martensi Karsch
文献类型：期刊论文
| 作者 | Cai, Z; Xu, CQ; Xu, YQ; Lu, WY; Chi, CW; Shi, YY; Wu, JH |
| 刊名 | BIOCHEMISTRY
 |
| 出版日期 | 2004 |
| 卷号 | 43期号:13页码:3764-3771 |
| 通讯作者 | Wu, JH (reprint author), Univ Sci & Technol China, Sch Life Sci, Struct Biol Lab, Hefei 230026, Anhui, Peoples R China.,wujihui@ustc.edu.cn |
| 英文摘要 | BmBKTx1 is a 31-amino acid peptide identified from the venom of the Chinese scorpion Buthus martensi Karsch, blocking high-conductance calcium-activated potassium channels. Sequence homology analysis indicates that BmBKTxl is a new subfamily of short-chain alpha-KTx toxins of the potassium channel, which we term alpha-KTx19. Synthetic BmBKTxl was prepared by using solid-phase peptide synthesis. Two-dimensional NMR spectroscopy techniques were used to determine the solution structure of BmBKTx1. The results show that the BmBKTx1 forms a typical cysteine-stabilized alpha/beta scaffold adopted by most short-chain scorpion toxins. The structure of BmBKTxl consists of a two-stranded antiparallel beta-sheet (residues 20-29) and an alpha-helix (residues 5-15). The three-dimensional structure of BmBKTxl was also compared with those of two function-related scorpion toxins, charybdotoxin (ChTx) and BmTx1, and their structural and functional implications are discussed. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ANDROCTONUS-MAURETANICUS-MAURETANICUS ; POTASSIUM CHANNEL ; K+ CHANNELS ; SHORT-CHAIN ; 3-DIMENSIONAL STRUCTURE ; PROTEIN STRUCTURES ; NMR-SPECTROSCOPY ; PEPTIDE LIGAND ; TOXINS ; VENOM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220580000002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2164]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Cai, Z,Xu, CQ,Xu, YQ,et al. Solution structure of BmBKTx1, a new BKCa1 channel blocker from the Chinese scorpion Buthus martensi Karsch[J]. BIOCHEMISTRY,2004,43(13):3764-3771. |
| APA | Cai, Z.,Xu, CQ.,Xu, YQ.,Lu, WY.,Chi, CW.,...&Wu, JH.(2004).Solution structure of BmBKTx1, a new BKCa1 channel blocker from the Chinese scorpion Buthus martensi Karsch.BIOCHEMISTRY,43(13),3764-3771. |
| MLA | Cai, Z,et al."Solution structure of BmBKTx1, a new BKCa1 channel blocker from the Chinese scorpion Buthus martensi Karsch".BIOCHEMISTRY 43.13(2004):3764-3771. |
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