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Interplay of SOX and POU factors in regulation of the Nestin gene in neural primordial cells
文献类型：期刊论文
| 作者 | Tanaka, S; Kamachi, Y; Tanouchi, A; Hamada, H; Jing, NH; Kondoh, H |
| 刊名 | MOLECULAR AND CELLULAR BIOLOGY
 |
| 出版日期 | 2004 |
| 卷号 | 24期号:20页码:8834-8846 |
| 通讯作者 | Kondoh, H (reprint author), Osaka Univ, Grad Sch Frontier Biosci, 1-3 Yamadaoka, Suita, Osaka 5650871, Japan.,j61056@hpc.cmc.osaka-u.ac.jp |
| 英文摘要 | Intermediate-filament Nestin and group B1 SOX transcription factors (SOX1/2/3) are often employed as markers for neural primordium, suggesting their regulatory link. We have identified adjacent and essential SOX and POU factor binding sites in the Nestin neural enhancer. The 30-bp sequence of the enhancer including these sites (Nes30) showed a nervous system-specific and SOX-POU-dependent enhancer activity in multimeric forms in transfection assays and was utilized in assessing the specificity of the synergism; combinations of either group B1 or group C SOX (SOX11) with class III POU proved effective. In embryonic day 13.5 mouse spinal cord, Nestin was expressed in the cells with nuclei in the ventricular and subventricular zones. SOX1/2/3 expression was confined to the nuclei of the ventricular zone; SOX11 localized to the nuclei of both subventricular (high-level expression) and intermediate (low-level expression) zones. Class III POU (Brn2) was expressed at high levels, localizing to the nucleus in the ventricular and subventricular zones; moderate expression was observed in the intermediate zone, distributed in the cytoplasm. These data support the model that synergic interactions between group B1/C SOX and class III POU within the nucleus determine Nestin expression. Evidence also suggests that such interactions are involved in the regulation of neural primordial cells. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | CENTRAL-NERVOUS-SYSTEM ; CNS STEM-CELLS ; TRANSCRIPTION FACTORS ; PROGENITOR CELLS ; CHICKEN SOX2 ; DNA-BINDING ; DOMAIN FACTORS ; EXPRESSION ; PROTEINS ; COMPLEX |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000224239100004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2177]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Tanaka, S,Kamachi, Y,Tanouchi, A,et al. Interplay of SOX and POU factors in regulation of the Nestin gene in neural primordial cells[J]. MOLECULAR AND CELLULAR BIOLOGY,2004,24(20):8834-8846. |
| APA | Tanaka, S,Kamachi, Y,Tanouchi, A,Hamada, H,Jing, NH,&Kondoh, H.(2004).Interplay of SOX and POU factors in regulation of the Nestin gene in neural primordial cells.MOLECULAR AND CELLULAR BIOLOGY,24(20),8834-8846. |
| MLA | Tanaka, S,et al."Interplay of SOX and POU factors in regulation of the Nestin gene in neural primordial cells".MOLECULAR AND CELLULAR BIOLOGY 24.20(2004):8834-8846. |
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