Characterization and promoter analysis of the mouse nestin gene
文献类型：期刊论文
| 作者 | Cheng, LP; Jin, ZG; Liu, L; Yan, Y; Li, TT; Zhu, XL; Jing, NH |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2004 |
| 卷号 | 565期号:1页码:195-202 |
| 关键词 | mouse nestin gene promoter Sp1 sp3 p19 cell |
| 通讯作者 | Jing, NH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Mol Cell Biol Lab, Lab Stem Cell Biol,Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,njing@sibs.ac.cn |
| 英文摘要 | The intermediate filament protein nestin is expressed in the neural stem cells of the developing central nervous system (CNS). Promoter analysis revealed that the minimal promoter of the mouse nestin gene resides in the region -11 to + 183 of the 5' non-coding and upstream flanking region, and that two adjacent Sp1-binding sites are necessary for promoter activity. Electrophoretic mobility-shift assays (EMSA) and supershift assays showed that Sp1 and Sp3 proteins selectively bind to the upstream Sp1 site. These results demonstrate an important functionality of Sp1 and Sp3 in regulating the expression of the mouse nestin gene. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | CENTRAL-NERVOUS-SYSTEM ; INTERMEDIATE FILAMENT PROTEIN ; TRANSCRIPTIONAL ACTIVATION ; SP1 BINDING ; STEM-CELLS ; IN-VIVO ; EXPRESSION ; RECEPTOR ; NEURONS ; RAT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000221397400037 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2196]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Cheng, LP,Jin, ZG,Liu, L,et al. Characterization and promoter analysis of the mouse nestin gene[J]. FEBS LETTERS,2004,565(1):195-202. |
| APA | Cheng, LP.,Jin, ZG.,Liu, L.,Yan, Y.,Li, TT.,...&Jing, NH.(2004).Characterization and promoter analysis of the mouse nestin gene.FEBS LETTERS,565(1),195-202. |
| MLA | Cheng, LP,et al."Characterization and promoter analysis of the mouse nestin gene".FEBS LETTERS 565.1(2004):195-202. |
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