Proteomic analysis of differential protein expression in a human hepatoma revertant cell line by using an improved two-dimensional electrophoresis procedure combined with matrix assisted laser desorption/ionization-time of flight-mass spectrometry
文献类型：期刊论文
| 作者 | Yuan, Q; An, J; Liu, DG; Sun, L; Ge, YZ; Huang, YL; Xu, GJ; Zhao, FK |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2004 |
| 卷号 | 25期号:1页码:1160-1168 |
| 关键词 | human tumorigenesis-related proteins mass spectrometry multiple strip one gel method two-dimensional polyacrylamide gel electrophoresis |
| 通讯作者 | Zhao, FK (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Prote, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,fkzhao@sibs.ac.cn |
| 英文摘要 | The proteomic profiles of a human hepatoma revertant, CL1, and its original cell line, SMMC7721, were compared by using an improved two-dimensional electrophoresis (2-DE) procedure, with multi-IPGstrips gels (length less than or equal to 13 cm) run simultaneously on one. sodium dodecyl sulfate (SDS) gel (shortened MSOG method). Nineteen proteins, showing significant difference in expression (P < 0.01), were selected and identified by matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and database search. In the revertant CL1 cells, compared to human hepatoma SMMC7721 cells, upregulated expression levels of some proteins related to tumor suppression, like maspin, were found, whereas some proteins related to tumor growth, like cathepsin D, were downregulated. These facts suggest that the phenotypic reversion of the CL1 cells was at least partially due to changes at the translational level of the proteins which favored the reconstruction of the normal phenotype of the cell. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 关键词[WOS] | IMMOBILIZED PH GRADIENTS ; HEPATOCELLULAR-CARCINOMA ; POLYACRYLAMIDE-GELS ; TUMOR-SUPPRESSOR ; CURRENT STATE ; CATHEPSIN-D ; MASPIN ; IDENTIFICATION ; GROWTH ; REPRODUCIBILITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000221168700029 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2200]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yuan, Q,An, J,Liu, DG,et al. Proteomic analysis of differential protein expression in a human hepatoma revertant cell line by using an improved two-dimensional electrophoresis procedure combined with matrix assisted laser desorption/ionization-time of flight-mass spectrometry[J]. ELECTROPHORESIS,2004,25(1):1160-1168. |
| APA | Yuan, Q.,An, J.,Liu, DG.,Sun, L.,Ge, YZ.,...&Zhao, FK.(2004).Proteomic analysis of differential protein expression in a human hepatoma revertant cell line by using an improved two-dimensional electrophoresis procedure combined with matrix assisted laser desorption/ionization-time of flight-mass spectrometry.ELECTROPHORESIS,25(1),1160-1168. |
| MLA | Yuan, Q,et al."Proteomic analysis of differential protein expression in a human hepatoma revertant cell line by using an improved two-dimensional electrophoresis procedure combined with matrix assisted laser desorption/ionization-time of flight-mass spectrometry".ELECTROPHORESIS 25.1(2004):1160-1168. |
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