Both N- and C-terminal regions are essential for cinnamomin A-chain to deadenylate ribosomal RNA and supercoiled double-stranded DNA
文献类型：期刊论文
| 作者 | He, WJ; Liu, WY |
| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2004 |
| 卷号 | 377期号:1页码:17-23 |
| 关键词 | cinnamomin A-chain deadenylation deletion mutation ribosome-inactivating protein supercoiled DNA |
| 通讯作者 | Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,wyliu@sibs.ac.cn |
| 英文摘要 | Cinnamomin is a type II ribosome-inactivating protein and its A-chain exhibits RNA N-glycosidase activity to remove an adenine in the conserved sarcin/ricin loop of the largest RNA in ribosome, arresting protein synthesis at the elongation step. In this report, deadenylation of both rRNA and supercoiled DNA by native and recombinant cinnamomin A-chain expressed in Escherichia coli was demonstrated. However, the mutants of cinnamomin A-chain devoid of N-terminal 52 or/and C-terminal 51 amino acid residues lost both the activity of RNA N-glycosidase and the ability to release adenines from supercoiled DNA. Additionally, supercoiled DNA could not be cleaved into nicked and linear forms by these mutants. These results indicate that both N- and C-terminal regions are essential for the cinnamomin A-chain to deadenylate rRNA and supercoiled DNA. It was suggested that phosphodiester bonds in the extensively deadenylated region of supercoiled DNA would become fragile and liable to be broken spontaneously owing to the existence of tension in the supercoiled DNA. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | INACTIVATING PROTEINS ; ACTIVE-SITE ; DEOXYRIBONUCLEASE ACTIVITY ; CAMPHOR TREE ; GELONIN ; RICIN ; SEEDS ; ANGSTROM ; PLANTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000187955200002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2211]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | He, WJ,Liu, WY. Both N- and C-terminal regions are essential for cinnamomin A-chain to deadenylate ribosomal RNA and supercoiled double-stranded DNA[J]. BIOCHEMICAL JOURNAL,2004,377(1):17-23. |
| APA | He, WJ,&Liu, WY.(2004).Both N- and C-terminal regions are essential for cinnamomin A-chain to deadenylate ribosomal RNA and supercoiled double-stranded DNA.BIOCHEMICAL JOURNAL,377(1),17-23. |
| MLA | He, WJ,et al."Both N- and C-terminal regions are essential for cinnamomin A-chain to deadenylate ribosomal RNA and supercoiled double-stranded DNA".BIOCHEMICAL JOURNAL 377.1(2004):17-23. |
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