Baculovirus p35 gene greatly enhances PC12 cell's resistance against oxidative stress
文献类型：期刊论文
| 作者 | Zhuo, M; Yu, FR; Xu, DH; Sun, LY; Liu, XY |
| 刊名 | JOURNAL OF THE NEUROLOGICAL SCIENCES
 |
| 出版日期 | 2003 |
| 卷号 | 216期号:1页码:135-141 |
| 关键词 | oxidative stress PC12 cell H2O2 6-OHDA baculovirus p35 gene |
| 通讯作者 | Liu, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Oxidative stress is thought to be a major contributor to the progress of the Parkinson's Disease (PD) because of the high vulnerability of dopaminergic cells against oxidative stress. The present work demonstrates that with the expression of the baculovirus p35 gene, PC12 cells could gain a high resistance against oxidative toxicants, hydrogen peroxide (H2O2) and 6-hydroxydopamine (6-OHDA). The DNA fragmentation analysis showed that PC12 cells underwent apoptosis after exposure to H2O2 or 6-OHDA, while PP35 cells, a p35-expressing PC12 cell line, did not. Flow cytometric analysis showed that treatment with 150 muM H2O2 or 120 muM 6-OHDA for 24 h caused 52.86% or 66.36% apoptotic cell, respectively, in PC12 cells, but only 4.26% or 5.80% in PP35 cells. The cell viability measured by 3-(4,5-dimethylthiazal-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay indicated that H2O2 and 6-OHDA induced a dose-dependent cell death on PC12 cells that were greatly remitted on PP35 cells. The viability of PP35 cells was even stronger than that of PC12 cells protected by glial cell line deprived neurotrophic factor (GDNF). The surviving PP35 cells remained normal cell morphology and showed positive with tyrosine hydroxylase (TH) immunocytochemical staining. These results indicate that baculovirus p35 gene possesses remarkable ability to rescue PC12 cells from death in experimental paradigms associated with oxidative stress. (C) 2003 Elsevier B.V. All rights reserved. |
| 学科主题 | Neurosciences & Neurology |
| 类目[WOS] | Clinical Neurology ; Neurosciences |
| 关键词[WOS] | ANTIAPOPTOTIC PROTEIN P35 ; CAENORHABDITIS-ELEGANS ; DOPAMINE NEURONS ; DEATH PROTEASE ; INHIBITION ; EXPRESSION ; APOPTOSIS ; GLUTATHIONE ; CLEAVAGE ; TOXICITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000186846600020 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2213]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhuo, M,Yu, FR,Xu, DH,et al. Baculovirus p35 gene greatly enhances PC12 cell's resistance against oxidative stress[J]. JOURNAL OF THE NEUROLOGICAL SCIENCES,2003,216(1):135-141. |
| APA | Zhuo, M,Yu, FR,Xu, DH,Sun, LY,&Liu, XY.(2003).Baculovirus p35 gene greatly enhances PC12 cell's resistance against oxidative stress.JOURNAL OF THE NEUROLOGICAL SCIENCES,216(1),135-141. |
| MLA | Zhuo, M,et al."Baculovirus p35 gene greatly enhances PC12 cell's resistance against oxidative stress".JOURNAL OF THE NEUROLOGICAL SCIENCES 216.1(2003):135-141. |
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