The functional domains of human ventricular myosin light chain 1
文献类型：期刊论文
| 作者 | Xie, BT; Huang, RJ; Huang, L; Zhou, GY; Gong, ZX |
| 刊名 | BIOPHYSICAL CHEMISTRY
 |
| 出版日期 | 2003 |
| 卷号 | 106期号:1页码:57-66 |
| 关键词 | actin ATPase activity binding human ventricular myosin light chain rat myosin S1 |
| 通讯作者 | Gong, ZX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The biological functions of the myosin light chain 1 (LC1) have not been clearly elucidated yet. In this work we cloned and expressed N- and C- terminal fragments of human ventricular LC1 (HVLC1) containing amino acid residues 1-98 and 99-195 and two parts, NN and NC of N fragment in GST-fusion forms, respectively. Using GST pull-down assay, the direct binding experiments of LC1 with rat cardiac G-actin, F-actin and thin filaments, as well as rat cardiac myosin heavy chain (RCMHC) have been performed. Furthermore, the recombinant complexes of rat myosin S I with N- and C-fragments, as well as the whole molecular of HVLC1 were generated. The results suggested that both binding sites of HVLC1 for actin and myosin heavy chain are positioned in its N-terminal fragment, which may contain several actin-binding sites in tandem. The polymerization of G-actin, the tropomyosin and troponin molecules located in the thin filaments do not hinder the binding of N-terminal fragment of HVLC1 with actin and thin filaments in vitro. The recombinant complex of rat cardiac myosin S1 (RCMS1) with N fragment of HVLC1 greatly decreased actin-actived Mg2+-ATPase activity for lack of C fragment. We conclude that the N-fragment is the binding domain of human ventricular LC1, whereas the C-fragment serves as a functional domain, which may be more involved in the modulation of the actin-activated ATPase activity of myosin. (C) 2003 Elsevier Science B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Chemistry |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Chemistry, Physical |
| 关键词[WOS] | SMOOTH-MUSCLE MYOSIN ; ATPASE ACTIVITY ; HEAVY-CHAIN ; BINDING-SITES ; ACTIN-BINDING ; N-TERMINUS ; SUBFRAGMENT-1 ; MOTOR ; GENE ; RECOMBINATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000185816200006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2215]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xie, BT,Huang, RJ,Huang, L,et al. The functional domains of human ventricular myosin light chain 1[J]. BIOPHYSICAL CHEMISTRY,2003,106(1):57-66. |
| APA | Xie, BT,Huang, RJ,Huang, L,Zhou, GY,&Gong, ZX.(2003).The functional domains of human ventricular myosin light chain 1.BIOPHYSICAL CHEMISTRY,106(1),57-66. |
| MLA | Xie, BT,et al."The functional domains of human ventricular myosin light chain 1".BIOPHYSICAL CHEMISTRY 106.1(2003):57-66. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。