Human mitochondrial leucyl-tRNA synthetase with high activity produced from Escherichia coli
文献类型：期刊论文
| 作者 | Yao, YN; Wang, L; Wu, XF; Wang, ED |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2003 |
| 卷号 | 30期号:1页码:112-116 |
| 关键词 | human mitochondrion aminoacyl-tRNA synthetase tRNA(Leu) mitochondria targeting presequence |
| 通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The processing of human mitochondrial leucyl-tRNA synthetase had been previously investigated in insect cell. In the present work, the gene encoding human mitochondrial leucyl-tRNA synthetase with the same N-terminus as that processed in the mitochondria of insect cell was cloned and expressed in Escherichia coli. The enzyme was purified by affinity chromatography on Ni-NTA column. About 6 mg of human mitochondrial leucyl-tRNA synthetase was obtained from 1 liter of culture. The specific activity of the purified enzyme is 127.7 units/mg, the highest activity of the reported results; this enzyme has the potential for characterizing the mitochondrial tRNA mutants associated with some human mitochondrion-related neuromuscular disorders. The kinetic constants for three substrates: leucine, ATP, and E coli tRNA(1)(leu) (CAG) in the leucylation reaction are also reported herein. (C) 2003 Elsevier Science (USA). All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | IDENTIFICATION ; RECOGNITION ; MUTATIONS ; TRNA(LEU) ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000183921700014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2220]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yao, YN,Wang, L,Wu, XF,et al. Human mitochondrial leucyl-tRNA synthetase with high activity produced from Escherichia coli[J]. PROTEIN EXPRESSION AND PURIFICATION,2003,30(1):112-116. |
| APA | Yao, YN,Wang, L,Wu, XF,&Wang, ED.(2003).Human mitochondrial leucyl-tRNA synthetase with high activity produced from Escherichia coli.PROTEIN EXPRESSION AND PURIFICATION,30(1),112-116. |
| MLA | Yao, YN,et al."Human mitochondrial leucyl-tRNA synthetase with high activity produced from Escherichia coli".PROTEIN EXPRESSION AND PURIFICATION 30.1(2003):112-116. |
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