Independency of anti-HIV-1 activity from ribosome-inactivating activity of trichosanthin
文献类型：期刊论文
| 作者 | Wang, JH; Nie, HL; Huang, H; Tam, SC; Zheng, YT |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2003 |
| 卷号 | 302期号:1页码:89-94 |
| 关键词 | trichosanthin ribosome inactivating protein human immunodeficiency virus type 1 antiviral protein |
| 通讯作者 | Zheng, YT (reprint author), Chinese Acad Sci, Kunming Inst Zool, Lab Mol Immunopharmacol, Kunming 650223, Peoples R China., |
| 英文摘要 | Trichosanthin (TCS) is a type I ribosome-inactivating (RI) protein possessing multiple biological and pharmacological activities. Its major action is inhibition of human immunodeficiency virus (HIV) replication but the mechanism is still elusive. All evidences showed that this action is related to its RI activity. Previous studies found that TCS mutants with reduced RI activity simultaneously lost some anti-HIV activity. In this study, an exception was demonstrated by two TCS mutants retaining almost all RI activity but were devoid of anti-HIV-1 activity. Five mutants were constructed by using site-directed mutagenesis with either deletion or addition of amino acids to the C-terminal sequence. Results showed that the RI activity of mutants with C-terminal deletion mutants (TCSC2, TCSC4, and TCSC14) decreased by 1.2-3.3-fold with parallel downshifting of its anti-HIV-1 activity (1.4-4.8-fold). Another two mutants, TCSC19aa and TCSKDEL having 19 amino acid extension and a KDEL signal sequence added to the C-terminal sequence, retained all RI activity but subsequently lost most of the anti-HIV-1 activity. These findings suggested that ribosome inactivation alone might not be adequate to explain the anti-HIV action of TCS. (C) 2003 Elsevier Science (USA). All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | SIGNAL-TRANSDUCTION ; PROTEIN ; INFLAMMATION ; RESIDUES ; GLQ223 ; AMINO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000181496900015 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2223]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, JH,Nie, HL,Huang, H,et al. Independency of anti-HIV-1 activity from ribosome-inactivating activity of trichosanthin[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2003,302(1):89-94. |
| APA | Wang, JH,Nie, HL,Huang, H,Tam, SC,&Zheng, YT.(2003).Independency of anti-HIV-1 activity from ribosome-inactivating activity of trichosanthin.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,302(1),89-94. |
| MLA | Wang, JH,et al."Independency of anti-HIV-1 activity from ribosome-inactivating activity of trichosanthin".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 302.1(2003):89-94. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。