IRBP-specific Th1 cells from peripheral blood were predominant in the experimental autoimmune uveitis
文献类型：期刊论文
| 作者 | Wu, YD; Lin, GM; Sun, B |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2003 |
| 卷号 | 302期号:1页码:150-155 |
| 关键词 | experimental autoimmune uveitis flow cytometry peripheral blood lymphocytes Th1 cell |
| 通讯作者 | Sun, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol Immunol & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | Experimental autoimmune uveitis (EAU) is a Th1-cell-mediated autoimmune disease. In this study, the correlation between IRBP-specific Th1 cells in PBLs and the histological grading in the eyes was evaluated kinetically during EAU induction. EAU was induced in B10.A mice with IRBP immunization and the eyes were enucleated for histological examination on days 0, 3, 7, 15, and 21 after immunization. To determine the Th1-cell-mediated immune response, Th1 cytokines (IL-12p40 and IFN-gamma) were measured by RT-PCR in inflamed eyes. At mean time, CD4(+) and IFN-gamma(+) double positive T cells (Th1 cells) from PBLs were analyzed by flow cytometry. The level of the IRBP-specific Th1 cells was significantly increased and kinetically changed during EAU induction, but the cells reached peak time early before the disease was onset. Those IRBP-specific Th1 cells in the PBLs were evidence for EAU disease, but its peak time was different from EAU disease in the eyes. Our data suggested that it is very important to collect blood from patients at a suitable time point and the Th1 cells measured by flow cytometry are good marker for disease diagnosis. (C) 2003 Elsevier Science (USA). All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | GENETIC SUSCEPTIBILITY ; DISEASE ; UVEORETINITIS ; EXPRESSION ; RAT ; EAU |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000181496900025 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2224]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wu, YD,Lin, GM,Sun, B. IRBP-specific Th1 cells from peripheral blood were predominant in the experimental autoimmune uveitis[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2003,302(1):150-155. |
| APA | Wu, YD,Lin, GM,&Sun, B.(2003).IRBP-specific Th1 cells from peripheral blood were predominant in the experimental autoimmune uveitis.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,302(1),150-155. |
| MLA | Wu, YD,et al."IRBP-specific Th1 cells from peripheral blood were predominant in the experimental autoimmune uveitis".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 302.1(2003):150-155. |
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