Preparation of an anti-Cdx-2 antibody for analysis of different species Cdx-2 binding to acat2 promoter
文献类型：期刊论文
| 作者 | Song, BL; Qi, W; Wang, CH; Yang, JB; Yang, XY; Lin, ZX; Li, BL |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:1页码:42167 |
| 关键词 | Cdx-2 GST fusion protein polyclonal antibody EMSA acat2 promoter |
| 通讯作者 | , |
| 英文摘要 | The homeodomain protein, Cdx-2, as transcription factor has been implicated in the transcriptional regulation of genes expressed in small intestine and the process of tumorgenesis. In current work, a conserved mouse Cdx-2 domain (mCdx-2D) coded by its cDNA fragment, which was amplified and cloned into the expression vector pGEX-4T-1, was expressed as a fusion protein with GST ( GST-mCdx-2D) and purified by one step of affinity chromatography. A polyclonal antibody against Cdx-2 was raised by using the recombinant fusion protein GST-mCdx-2D as antigen and was fractionated from the rabbit anti-serum. Western blot and EMSA ( electrophoretic mobility shift assay) demonstrate that the natural and denatured Cdx-2s from different species ( mouse and human) can be detected by the prepared anti-Cdx-2 antibody. Most notably, we found that the Cdx-2 in human intestine cell line Caco-2 is expressed in a differentiation-dependent manner and can efficiently bind to the mouse and human acat2 ( acyl-coenzyme A: cholesterol acyltransferase 2) promoter regions, suggesting that the transcriptional factor Cdx-2 may play a role in regulating the acat2 expression in the intestinal cells. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ACYL-COENZYME-A ; COA-CHOLESTEROL ACYLTRANSFERASE ; HOMEOBOX GENE CDX-2 ; CALBINDIN-D9K GENE ; TRANSCRIPTIONAL ACTIVATION ; INTESTINAL EPITHELIUM ; PROTEIN CDX-2/3 ; DOWN-REGULATION ; HUMAN LIVER ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000180539500002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2227]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Song, BL,Qi, W,Wang, CH,et al. Preparation of an anti-Cdx-2 antibody for analysis of different species Cdx-2 binding to acat2 promoter[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(1):42167. |
| APA | Song, BL.,Qi, W.,Wang, CH.,Yang, JB.,Yang, XY.,...&Li, BL.(2003).Preparation of an anti-Cdx-2 antibody for analysis of different species Cdx-2 binding to acat2 promoter.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(1),42167. |
| MLA | Song, BL,et al."Preparation of an anti-Cdx-2 antibody for analysis of different species Cdx-2 binding to acat2 promoter".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.1(2003):42167. |
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