Expression, purification and in vitro N-myristoylation of human Src N-terminal region
文献类型：期刊论文
| 作者 | Ma, HH; Yang, L; Li, BL |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:1页码:13-17 |
| 关键词 | Src His-tag fusion expression N-myristoylation |
| 通讯作者 | , |
| 英文摘要 | The DNA fragment encoding N-terminal region of human c-Src was amplified from Caco-2 cell total RNA by RT-PCR and cloned into vector pMFHT to obtain His-tag fusion expression plasmid pMF-SrcHT, which was based on 17 expression system. The fusion protein SrcHT was highly expressed in E. coli BL21 (DE3) harboring the pMF-SrcHT and purified from bacterial lysate by Ni-IDA affinity chromatography. The assays using [H-3]-labeled substrate demonstrate that the purified fusion protein SrcHT can be effectively N-myristoylated by recombinant human myristoyl-CoA: protein N-myristoyltransferase ( NMT) in vitro. This work is a basis for further biochemical studies and development of new anti-cancer chemotherapeutic drugs based on specific inhibition of N-myristoylation of human Src. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PROTEIN-KINASE ; FUSION EXPRESSION ; MYRISTOYLTRANSFERASE ; ACYLATION ; P60SRC ; COA ; RAT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000180539500003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2228]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ma, HH,Yang, L,Li, BL. Expression, purification and in vitro N-myristoylation of human Src N-terminal region[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(1):13-17. |
| APA | Ma, HH,Yang, L,&Li, BL.(2003).Expression, purification and in vitro N-myristoylation of human Src N-terminal region.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(1),13-17. |
| MLA | Ma, HH,et al."Expression, purification and in vitro N-myristoylation of human Src N-terminal region".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.1(2003):13-17. |
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