Involvement of PHO80 and PHO85 genes in Saccharomyces cerevisiae ion tolerance
文献类型：期刊论文
| 作者 | Mao, XC; Xia, YL; Hu, YF; Lu, CD |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:1页码:86-91 |
| 关键词 | PHO85 PHO80 metal ion tolerance |
| 通讯作者 | , |
| 英文摘要 | PH085 is a versatile gene in Saccharomyces cerevisiae, which is involved in metabolism of inorganic phosphate and usage of carbon source, accumulation of glycogen, regulation of protein stability and cell cycle control. The viability of wild type budding yeast strain YPH499 and its derivative pho85Delta mutant, pho80Delta mutant, and pap1 (pcl7)Delta mutant in different cations were investigated and their tolerance to the cations(LC50) was measured. The results showed that the deletion of PHO85 or PHO80 gene both increased sensibility of Sacchromyces cerevisiae to ions K+, Mg2+, Zn2+, Ca2+ and Mn2+, while the deletion of pap1 (pcl7) gene did not lead to such phenotype. The difference between the patterns of relative growth curve of the mutants and wild type strain in the above ions also implied that PHO80 was the unique PCLs in complex with PH085 CDK, that were contributed to K+ and Mg2+ ion homeostasis control and there were some other PCLs besides PHO80 that were involved in Zn, Ca and Mn2+ tolerance regulation as cyclin of PH085 CDK. Furthermore, the amount of the total cellular calcium of pho85Delta mutant, pho80Delta mutant and YPH499 indicated that the ability of calcium accumulation of pho85Delta mutant and pho80Delta mutant was impaired. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | CYCLIN-DEPENDENT KINASE ; VACUOLAR H+-ATPASE ; PROTEIN-KINASE ; CELL-CYCLE ; PHOSPHATASE CALCINEURIN ; CA-2+ INFLUX ; YEAST-CELLS ; CDK COMPLEX ; PHOSPHORYLATION ; HOMEOSTASIS |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000180539500015 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2231]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Mao, XC,Xia, YL,Hu, YF,et al. Involvement of PHO80 and PHO85 genes in Saccharomyces cerevisiae ion tolerance[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(1):86-91. |
| APA | Mao, XC,Xia, YL,Hu, YF,&Lu, CD.(2003).Involvement of PHO80 and PHO85 genes in Saccharomyces cerevisiae ion tolerance.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(1),86-91. |
| MLA | Mao, XC,et al."Involvement of PHO80 and PHO85 genes in Saccharomyces cerevisiae ion tolerance".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.1(2003):86-91. |
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