Differentiation of velogenic, mesogenic and lentogenic strains of Newcastle disease virus by multiplex RT PCR
文献类型：期刊论文
| 作者 | Shan, SH; Shao, CG; Xu, CZ; Zou, J; Hu, YQ; Wu, JH; Gong, ZX |
| 刊名 | ANNALS OF APPLIED BIOLOGY
 |
| 出版日期 | 2003 |
| 卷号 | 142期号:1页码:49-54 |
| 关键词 | Newcastle disease virus pathotypes multiplex RT PCR |
| 通讯作者 | Gong, ZX (reprint author), Acad Sinica, Shanghai Inst Biochem & Cell Biol, Key Lab Proteom, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | A multiplex RT-PCR technique has been developed for differentiation of velogenic, mesogenic and lentogenic pathotypes of Newcastle disease virus (NDV), using a set of three oligonucleotide primers designed from NDV genomic RNA (P1, P2 and P3). The primer pair P1 and P2 generated a RT-PCR product of 204 bp, only with RNA from velogenic and mesogenic strains, whereas the P1 and P3 generated a 364 by product only with RNA from mesogenic and lentogenic strains. Thirty four NDV strains, including some reference strains (known pathotypes), NDV field isolates and NDV vaccine strains, as well as other avian virus strains, were tested with multiplex RT-PCR. All reference strains tested were differentiated in agreement with their intracerebral pathogenicity index (ICPI) values or with the pathotypes known in previous reports. The nucleotide sequence analysis of RT-PCR products for four NDV strains was fully in agreement with the RT-PCR characterisations of these strains. The RTPCR results of other avian RNA viruses further confirmed the reliability and specificity of this technique. However, the RTPCR failed to detect some other avian NDV which may not originate from chicken. This multiplex RT-PCR technique is simple and easy to perform. It could be applied not only to determine the origin of NDV but also may be used diagnostically in molecular epidemiological analysis of ND and for prediction of pathotypes of NDV isolates. |
| 学科主题 | Agriculture |
| 类目[WOS] | Agriculture, Multidisciplinary |
| 关键词[WOS] | POLYMERASE CHAIN-REACTION ; ASSAY ; SEQUENCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000181317500007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2232]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Shan, SH,Shao, CG,Xu, CZ,et al. Differentiation of velogenic, mesogenic and lentogenic strains of Newcastle disease virus by multiplex RT PCR[J]. ANNALS OF APPLIED BIOLOGY,2003,142(1):49-54. |
| APA | Shan, SH.,Shao, CG.,Xu, CZ.,Zou, J.,Hu, YQ.,...&Gong, ZX.(2003).Differentiation of velogenic, mesogenic and lentogenic strains of Newcastle disease virus by multiplex RT PCR.ANNALS OF APPLIED BIOLOGY,142(1),49-54. |
| MLA | Shan, SH,et al."Differentiation of velogenic, mesogenic and lentogenic strains of Newcastle disease virus by multiplex RT PCR".ANNALS OF APPLIED BIOLOGY 142.1(2003):49-54. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。