Purification of two depressant insect neurotoxins and their gene cloning from the scorpion Buthus martensi Karsch
文献类型：期刊论文
| 作者 | Wang, CG; Ling, MH; Chi, CW; Wang, DC; Stankiewicz, M; Pelhate, M |
| 刊名 | JOURNAL OF PEPTIDE RESEARCH
 |
| 出版日期 | 2003 |
| 卷号 | 61期号:1页码:42201 |
| 关键词 | amidation depressant insect toxin electrophysiological activity RACE scorpion |
| 通讯作者 | Chi, CW (reprint author), Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Insect-specific neurotoxins are important components of scorpion venoms. In this study, two toxins from the scorpion Buthus martensi Karsch (BmK) were purified. They shared high sequence homology with other depressant insect toxins and were designated BmK ITa and BmK lTb, respectively. They were able to suppress the action potential of cockroach isolated axon, which is due to a decrease in the peak sodium current. Furthermore, the effect of BmK ITb was lower than that of BmK ITa, and some of the electrophysiological characteristics of BmK ITb even resemble that of excitatory insect toxins. Their primary structures were determined by N-terminal partial sequence determination and cDNA cloning. The differences in their structures, especially the 31st residues, may result in the unique activity of BmK ITb. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 关键词[WOS] | ANDROCTONUS-AUSTRALIS HECTOR ; CA2+-ACTIVATED K+ CHANNELS ; SELECTIVE NEUROTOXIN ; POTASSIUM CHANNELS ; BUTHOTUS-JUDAICUS ; TITYUS-SERRULATUS ; SODIUM-CHANNEL ; CDNA SEQUENCE ; VENOM ; TOXIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000180332800002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2237]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, CG,Ling, MH,Chi, CW,et al. Purification of two depressant insect neurotoxins and their gene cloning from the scorpion Buthus martensi Karsch[J]. JOURNAL OF PEPTIDE RESEARCH,2003,61(1):42201. |
| APA | Wang, CG,Ling, MH,Chi, CW,Wang, DC,Stankiewicz, M,&Pelhate, M.(2003).Purification of two depressant insect neurotoxins and their gene cloning from the scorpion Buthus martensi Karsch.JOURNAL OF PEPTIDE RESEARCH,61(1),42201. |
| MLA | Wang, CG,et al."Purification of two depressant insect neurotoxins and their gene cloning from the scorpion Buthus martensi Karsch".JOURNAL OF PEPTIDE RESEARCH 61.1(2003):42201. |
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