Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus
文献类型：期刊论文
| 作者 | Sun, HF; Luo, HB; Yu, CY; Sun, T; Chen, J; Peng, SY; Qin, J; Shen, JH; Yang, YM; Xie, YH |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2003 |
| 卷号 | 32期号:2页码:302-308 |
| 关键词 | Severe Acute Respiratory Syndrome (SARS) SARS coronavirus 3C like protease molecular cloning expression purification mass spectrometric characterization |
| 通讯作者 | Jiang, HL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res,Drug Discovery & Design Ct, Shanghai 201203, Peoples R China., |
| 英文摘要 | Severe acute respiratory syndrome (SARS) is an acute respiratory illness, which has broken out in China. It has been known that SARS coronavirus (SARS_CoV) is a novel human coronavirus and is responsible for SARS infection. Belonging to one of the major proteins associated with SARS_CoV, SARS 3C-like protease (SARS_3CL(pro)) functions as a cysteine protease engaging in the proteolytic cleavage of the viral precursor polyprotein to a series of functional proteins required for coronavirus replication and is considered as an appealing target for designing anti-SARS agents. To facilitate the studies regarding the functions and structures of SARS_3CL(pro), in this report the synthetic genes encoding 3CL(pro), of SARS_CoV were assembled, and the plasmid was constructed using pQE30 as vector and expressed in Escherichia coli M15 cells. The highly yielded (similar to15 mg/L) expressed protease was purified by use of NTA-Ni2+ affinity chromatography and FPLC system, and its sequence was determined by LC/MS with the residue coverage of 46.4%. (C) 2003 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | ACUTE RESPIRATORY SYNDROME ; VIRUS-ENCODED PROTEINASES ; RNA-POLYMERASE ; SEQUENCE ; IDENTIFICATION ; POLYPROTEIN ; KILOBASES ; DESIGN ; GENE-1 ; DOMAIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000187393600017 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2242]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Sun, HF,Luo, HB,Yu, CY,et al. Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus[J]. PROTEIN EXPRESSION AND PURIFICATION,2003,32(2):302-308. |
| APA | Sun, HF.,Luo, HB.,Yu, CY.,Sun, T.,Chen, J.,...&Jiang, HL.(2003).Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus.PROTEIN EXPRESSION AND PURIFICATION,32(2),302-308. |
| MLA | Sun, HF,et al."Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus".PROTEIN EXPRESSION AND PURIFICATION 32.2(2003):302-308. |
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