Affinity and kinetics of P-selectin binding to heparin
文献类型：期刊论文
| 作者 | Wang, JG; Geng, JG |
| 刊名 | THROMBOSIS AND HAEMOSTASIS
 |
| 出版日期 | 2003 |
| 卷号 | 90期号:2页码:309-316 |
| 关键词 | P-selectin heparin heparan sulfate proteoglycans affinity kinetics |
| 通讯作者 | Geng, JG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Cell Biol Bldg Room 505,320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | P-selectin (CD62P), expressed on stimulated endothelial cells and activated platelets, reacts with P-selectin glycoprotein ligand-I (PSGL-1, CD 162) for leukocyte rolling. It also binds to heparin and heparan sulfate proteoglycans (HSPGs), which attenuates P-selectin mediated adhesions of leukocytes and cancer cells. Here we report that P-selectin mediated adhesion, but not rolling, of the HSPGs bearing human malignant melanoma A375 cells under shear Stress. To understand its underlying molecular mechanism, we measured the biophysical properties of this interaction. Heparin inhibited the adhesion of A375 cells to immobilized P-selectin under flow (IC50 = 3 muM heparin) and neutralized the binding of P-selectin to A375 cells (IC50 = 4 muM heparin). Using surface plasmon resonance technique, we found that P-selectin bound to heparin with a dissociation constant (K-d) of 115 +/- 6 nM. The measured off rate (k(off)) was 3.15 +/- 0.34 x 10(-3) s(-1) and the calculated on rate (k(on)) was 2.75 x 10(4) M-1 s(-1). Taken together, our data suggest that the very slow k(off) and the reduced k(on), but apparently not the K-d, are responsible for adhesion, but not rolling of A375 cells, to P-selectin under flow. |
| 学科主题 | Hematology; Cardiovascular System & Cardiology |
| 类目[WOS] | Hematology ; Peripheral Vascular Disease |
| 关键词[WOS] | CELL-ADHESION ; ACUTE-INFLAMMATION ; ENDOTHELIAL-CELLS ; ROLLING ADHESION ; FLOW CONDITIONS ; SULFATE ; LIGANDS ; PROTEOGLYCANS ; CONNECTIONS ; PLATELETS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184774000018 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2246]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, JG,Geng, JG. Affinity and kinetics of P-selectin binding to heparin[J]. THROMBOSIS AND HAEMOSTASIS,2003,90(2):309-316. |
| APA | Wang, JG,&Geng, JG.(2003).Affinity and kinetics of P-selectin binding to heparin.THROMBOSIS AND HAEMOSTASIS,90(2),309-316. |
| MLA | Wang, JG,et al."Affinity and kinetics of P-selectin binding to heparin".THROMBOSIS AND HAEMOSTASIS 90.2(2003):309-316. |
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