Complete sequence of the 10.3 kb silkworm Attacus ricini rDNA repeat, determination of the transcriptional initiation site and functional analysis of the intergenic spacer
文献类型：期刊论文
| 作者 | Wang, SQ; Zhao, MJ; Li, TP |
| 刊名 | DNA SEQUENCE
 |
| 出版日期 | 2003 |
| 卷号 | 14期号:2页码:95-101 |
| 关键词 | rRNA genes silkworm Attacus ricini transcription initiation site intergenic spacer |
| 通讯作者 | Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | A complete sequence of the 10.3 kb silkworm rDNA repeat in Attacus ricini (also called as Samia cynthia ricini) was first determined here, and sequences coding for 18S, 5.8S and 28S were also mapped to rDNA unit. The precise transcription initiation site was determined by primer extension experiment. Intergenic spacer (IGS) in the length of 2670 by in which transcription of 45s rRNA precursor starts was found to contain three distinct AT rich (A + T > 75%) regions. The AT richest region (from -320 to -3, A + T > 83%) is just in front of the transcription initiation site. Many short sequence homologies were also found to exist in the transcription initiation region. It clearly demonstrates that the nucleotide sequence of initiation region of rRNA shows great diversity among different species, but the general functional architectures show some conservation. Analyzing the IGS region of A. ricini may provide new insights into the mechanisms of the expression regulation of RNA polymerase I contributed genes. |
| 学科主题 | Biotechnology & Applied Microbiology; Genetics & Heredity |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 关键词[WOS] | RIBOSOMAL-RNA GENE ; DROSOPHILA-MELANOGASTER ; DNA ; REGION ; INVITRO ; REPLICATION ; PROMOTERS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000182082000003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2250]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, SQ,Zhao, MJ,Li, TP. Complete sequence of the 10.3 kb silkworm Attacus ricini rDNA repeat, determination of the transcriptional initiation site and functional analysis of the intergenic spacer[J]. DNA SEQUENCE,2003,14(2):95-101. |
| APA | Wang, SQ,Zhao, MJ,&Li, TP.(2003).Complete sequence of the 10.3 kb silkworm Attacus ricini rDNA repeat, determination of the transcriptional initiation site and functional analysis of the intergenic spacer.DNA SEQUENCE,14(2),95-101. |
| MLA | Wang, SQ,et al."Complete sequence of the 10.3 kb silkworm Attacus ricini rDNA repeat, determination of the transcriptional initiation site and functional analysis of the intergenic spacer".DNA SEQUENCE 14.2(2003):95-101. |
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