CDNA cloning and characterization of Agkistin, a new metalloproteinase from Agkistrodon halys
文献类型：期刊论文
| 作者 | Wang, SH; Shen, XC; Yang, GZ; Wu, XF |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2003 |
| 卷号 | 301期号:2页码:298-303 |
| 关键词 | SVMP disintegrin platelet aggregation apoptosis angiogenesis |
| 通讯作者 | Wu, XF (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Shanghai Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Agkistin was a new snake venom metalloproteinase (SVMP) gene which was cloned from Agkistrodon halys. Its deduced amino acid sequence has two additional cysteines (Cys407 and Cys426) in the disintegrin domain compared to other RGD containing SVMPs. The full-length gene (Agkistin) and its disintegrin region (named Agkistin-s) were expressed by baculovirus expression system (pFastBac-Htb vector) with His-tag, and their platelet aggregation-inhibition activity was evaluated. The expressed protein Agkistin can also induce apoptosis of HMEC cells in the basal medium after incubated at 37degreesC for 20 h. (C) 2003 Elsevier Science (USA). All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | THROMBIN-LIKE ENZYME ; SNAKE-VENOM ; ENDOTHELIAL-CELLS ; DISINTEGRIN ; APOPTOSIS ; PURIFICATION ; RHODOSTOMIN ; EXPRESSION ; SEQUENCE ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000181099100007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2251]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, SH,Shen, XC,Yang, GZ,et al. CDNA cloning and characterization of Agkistin, a new metalloproteinase from Agkistrodon halys[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2003,301(2):298-303. |
| APA | Wang, SH,Shen, XC,Yang, GZ,&Wu, XF.(2003).CDNA cloning and characterization of Agkistin, a new metalloproteinase from Agkistrodon halys.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,301(2),298-303. |
| MLA | Wang, SH,et al."CDNA cloning and characterization of Agkistin, a new metalloproteinase from Agkistrodon halys".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 301.2(2003):298-303. |
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