Secretory expression of the superactive [Lys(17,18), Glu(21)]-glucagon in E-coli
文献类型：期刊论文
| 作者 | Wen, CW; Gan, RB; Zhu, SQ |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:2页码:167-171 |
| 关键词 | genetical engineering glucagon SA-glucagon P-L promoter secretion |
| 通讯作者 | , |
| 英文摘要 | One of the most important findings in structure-function studies on glucagon by means of chemical synthesis is the discovery that [Lys(17,18), Glu(21)] glucagon had higher biological activity than native mutant of glucagon was called superactive glucagon (SA-glucagon). In the present work, the possibility to obtain SA-glucagon by means of genetic engineering was studied. The gene of SA-glucagon (SAG) was obtained by PCR from a constructed recombinant glucagon plasmid, pAGluT. A secretory expression vector harboring SAG, pBLSG7, containing P-l promoter and the gene of phoA signal peptide was constructed. In expression studies after transformation of pBLSG7 into E. coli BL21, it was found that the expression yield of SA-glucagon reached 3.65 mg/L(A600 = 1), about 19.5% of total proteins in the culture medium under shaken flask conditions. In addition, the influence of induction temperature and of E. coli strain on the expression yield of SA-glucagon was also studied. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ESCHERICHIA-COLI ; ALKALINE-PHOSPHATASE ; GLUCAGON ; GENE |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000181245500012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2254]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wen, CW,Gan, RB,Zhu, SQ. Secretory expression of the superactive [Lys(17,18), Glu(21)]-glucagon in E-coli[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(2):167-171. |
| APA | Wen, CW,Gan, RB,&Zhu, SQ.(2003).Secretory expression of the superactive [Lys(17,18), Glu(21)]-glucagon in E-coli.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(2),167-171. |
| MLA | Wen, CW,et al."Secretory expression of the superactive [Lys(17,18), Glu(21)]-glucagon in E-coli".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.2(2003):167-171. |
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