Synthesis of osteogenic growth peptide and its synergetic effect with granulocyte colony stimulating factor
文献类型：期刊论文
| 作者 | Liu, ZH; Shao, YC; Chen, HH; Shi, DY; Chen, TY; Cui, DF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:2页码:177-181 |
| 关键词 | OGP G-CSF synergy hematopoiesis solid phase synthesis |
| 通讯作者 | , |
| 英文摘要 | Osteogenic growth peptide (OGP) has been synthesized through Fmoc solid phase synthesis procedure. The purity of synthetic OGP (sOGP) is over 98.6% identified by HPLC, the amino acid sequence and electro-spray mass spectroscopy are consistent with theoretical values. The synergetic effect of sOGP with recombinant human granulocyte-colony stimulating factor (rhG-CSF) on the hematopoiesis was investigated in normal mice. To assess the synergy of sOGP with rhG-CSF, two schemes were designed. In one scheme rhG-CSF was used at the last 8 days of a 13-day treatment with sOGP, while in the other one both cytokines were given concurrently for 10 days [ sOGP, 0.5 nmol/day (mouse); rhG-CSF, 2 mug/day ( mouse)]. Both schemes showed that sOGP remarkably synergized with rhG-CSF on increment of white blood cell number and lymphocyte number in peripheral blood without any change of red blood cell and platelet counts. Quantitative differential analysis of bone marrow and histological examination of the spleen and sternum showed that sOGP plus rhG-CSF did not cause abnormal hyperplasia, so sOGP is a very hopeful new drug to improve the effectiveness of clinical used rhG-CSF. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | CARBOXY-TERMINAL PENTAPEPTIDE ; HEMATOPOIETIC STEM-CELLS ; BONE-MARROW INTERACTIONS ; HEMATOLOGICAL ACTIVITY ; PROGENITOR CELLS ; FLT3 LIGAND ; BLOOD ; MICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000181245500014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2255]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, ZH,Shao, YC,Chen, HH,et al. Synthesis of osteogenic growth peptide and its synergetic effect with granulocyte colony stimulating factor[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(2):177-181. |
| APA | Liu, ZH,Shao, YC,Chen, HH,Shi, DY,Chen, TY,&Cui, DF.(2003).Synthesis of osteogenic growth peptide and its synergetic effect with granulocyte colony stimulating factor.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(2),177-181. |
| MLA | Liu, ZH,et al."Synthesis of osteogenic growth peptide and its synergetic effect with granulocyte colony stimulating factor".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.2(2003):177-181. |
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