Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources
文献类型：期刊论文
| 作者 | Xie, JL; Zhang, L; Ye, Q; Zhou, QW; Xin, L; Du, P; Gan, RB |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2003 |
| 卷号 | 25期号:2页码:173-177 |
| 关键词 | angiostatin dissolved O-2 glycerol feeding Pichia pastoris |
| 通讯作者 | Ye, Q (reprint author), E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China., |
| 英文摘要 | A recombinant strain of Pichia pastoris with a phenotype of Mut(S) was used to produce angiostatin. Due to the low methanol consumption rate of this strain, both methanol and glycerol feedings, that produced oscillation in dissolved O-2 concentration, were used during the expression phase to improve cell growth and angiostatin expression. However, enhanced cell growth led to nitrogen limitation that suppressed further production of angiostatin, but addition of ammonia allowed angiostatin concentration to reach 108 mg l(-1) after an expression period of 96 h. The ratio of consumed glycerol to methanol of 1.5:1 (w/w) in the expression phase suggested that methanol played an important role in the metabolism of carbon sources. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | FERMENTATION ; PROTEIN ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000180344400015 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2259]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xie, JL,Zhang, L,Ye, Q,et al. Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources[J]. BIOTECHNOLOGY LETTERS,2003,25(2):173-177. |
| APA | Xie, JL.,Zhang, L.,Ye, Q.,Zhou, QW.,Xin, L.,...&Gan, RB.(2003).Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources.BIOTECHNOLOGY LETTERS,25(2),173-177. |
| MLA | Xie, JL,et al."Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources".BIOTECHNOLOGY LETTERS 25.2(2003):173-177. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。