Construction of secretory expression system suitable to express glucagon under the control of P-L promoter
文献类型：期刊论文
| 作者 | Wen, CW; Gan, RB; Zhu, SQ |
| 刊名 | CURRENT MICROBIOLOGY
 |
| 出版日期 | 2003 |
| 卷号 | 47期号:3页码:180-185 |
| 通讯作者 | Zhu, SQ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | It was reported that P-L promoter and alkaline phosphatase (phoA) signal peptide were used to construct secretory expression plasmid suitable to express glucagon and [Des-His(1)] glucagon in E. coli BL21 herein. Expression studies showed these two peptides could be expressed and secreted into the culture medium. The expression yield of recombinant glucagon reached 3.46 mg/L/OD600 unit of cells in shake flask. The yield of [Des-His(1)] glucagon was found to be higher than that of glucagon. In addition, some factors involved in secretion were studied too. |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 关键词[WOS] | RECOMBINANT HUMAN GLUCAGON ; COLI ALKALINE-PHOSPHATASE ; ESCHERICHIA-COLI ; SIGNAL PEPTIDE ; TERMINAL REGION ; FUSION PROTEIN ; SEQUENCE ; EXPORT ; HYDROPHOBICITY ; HORMONE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184714600003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2261]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wen, CW,Gan, RB,Zhu, SQ. Construction of secretory expression system suitable to express glucagon under the control of P-L promoter[J]. CURRENT MICROBIOLOGY,2003,47(3):180-185. |
| APA | Wen, CW,Gan, RB,&Zhu, SQ.(2003).Construction of secretory expression system suitable to express glucagon under the control of P-L promoter.CURRENT MICROBIOLOGY,47(3),180-185. |
| MLA | Wen, CW,et al."Construction of secretory expression system suitable to express glucagon under the control of P-L promoter".CURRENT MICROBIOLOGY 47.3(2003):180-185. |
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