HCRP1, a novel gene that is downregulated in hepatocellular carcinoma, encodes a growth-inhibitory protein
文献类型：期刊论文
| 作者 | Xu, ZH; Liang, L; Wang, HF; Li, TP; Zhao, MJ |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2003 |
| 卷号 | 311期号:4页码:1057-1066 |
| 关键词 | cDNA growth inhibitor colony formation hepatoma RNA interference |
| 通讯作者 | Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | One of the most frequent allelic deletions in hepatocellular carcinoma (HCC) has been found at chromosome 8p21-23. We reported here the identification and characterization of a novel gene for a hepatocellular carcinoma related protein 1 (HCRP1) localized at 8p22, which was isolated by positional candidate cloning. The expression of the gene for HCRP1 was most abundant in normal human liver tissue and significantly reduced or undetected in HCC tissues. The analysis of subcellular distribution showed that HCRP1 diffused in the cytoplasm with a significant fraction accumulated in the nuclei. After introduction of the sense and antisense cDNA of HCRP1 into HCC cell line SMMC-7721, we observed that the overexpression of HCRP1 significantly inhibited both anchorage-dependent and anchorage-independent cell growth in vitro. Using the transgenic short hairpin RNA (shRNA) to knock down the expression of HCRP1 gene in the other HCC cell line BEL-7404 resulted in the cell growth greatly enhanced. Moreover, reduction of the HCRP1 gene expression could also elevate the invasive ability of BEL-7404 cells. Our results strongly suggest that HCRP1 might be a growth inhibitory protein and associated with decreasing the invasion of HCC cells. (C) 2003 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | TUMOR-SUPPRESSOR GENES ; CHROMOSOME 8P ; LUNG-CANCER ; HUMAN-CELLS ; SHORT ARM ; HETEROZYGOSITY ; IDENTIFICATION ; LOCALIZATION ; DLC-1 ; LOCI |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000186803900039 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2272]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, ZH,Liang, L,Wang, HF,et al. HCRP1, a novel gene that is downregulated in hepatocellular carcinoma, encodes a growth-inhibitory protein[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2003,311(4):1057-1066. |
| APA | Xu, ZH,Liang, L,Wang, HF,Li, TP,&Zhao, MJ.(2003).HCRP1, a novel gene that is downregulated in hepatocellular carcinoma, encodes a growth-inhibitory protein.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,311(4),1057-1066. |
| MLA | Xu, ZH,et al."HCRP1, a novel gene that is downregulated in hepatocellular carcinoma, encodes a growth-inhibitory protein".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 311.4(2003):1057-1066. |
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