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Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes
文献类型：期刊论文
| 作者 | Chen, Y; He, ZX; Liu, AL; Wang, K; Maoi, WW; Chu, JX; Lu, Y; Fang, ZF; Shi, YT; Yang, QZ |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2003 |
| 卷号 | 13期号:4页码:251-263 |
| 关键词 | nuclear transfer (NT) somatic cell nuclear transfer (SCNT) embryonic stem cells (ES cell) therapeutic cloning rabbit oocyte |
| 通讯作者 | Sheng, HZ (reprint author), Shanghai Med Univ 2, Xinhua Hosp, Ctr Dev Biol, 1665 Kong Jiang Rd, Shanghai 200092, Peoples R China.,hzsheng@sh163a.sta.net.cn |
| 英文摘要 | To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PCR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well as mixed cell populations that express markers representative of all three germ layers. Thus, ntES cells derived from human somatic cells by NT to rabbit eggs retain phenotypes similar to those of conventional human ES cells, including the ability to undergo multilineage cellular differentiation. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | THERAPEUTIC CLONING ; HUMAN BLASTOCYSTS ; IN-VITRO ; LINES ; DIFFERENTIATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000185166200005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2275]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, Y,He, ZX,Liu, AL,et al. Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes[J]. CELL RESEARCH,2003,13(4):251-263. |
| APA | Chen, Y.,He, ZX.,Liu, AL.,Wang, K.,Maoi, WW.,...&Sheng, HZ.(2003).Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes.CELL RESEARCH,13(4),251-263. |
| MLA | Chen, Y,et al."Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes".CELL RESEARCH 13.4(2003):251-263. |
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